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Application of erigeron breviscapus extract in preparing anti-tumor or angiogenesis-suppressing medicaments, health-care food or cosmetics

A technology of angiogenesis and breviscapine, which is applied in the direction of antineoplastic drugs, cosmetic preparations, applications, etc., to achieve the effect of inhibiting tumor and tumor angiogenesis

Inactive Publication Date: 2010-09-15
苏州中药研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the investigation of the existing technology, there is no relevant literature report on the inhibitory effect of Erigeron breviscapine extract on tumor and tumor angiogenesis

Method used

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  • Application of erigeron breviscapus extract in preparing anti-tumor or angiogenesis-suppressing medicaments, health-care food or cosmetics
  • Application of erigeron breviscapus extract in preparing anti-tumor or angiogenesis-suppressing medicaments, health-care food or cosmetics
  • Application of erigeron breviscapus extract in preparing anti-tumor or angiogenesis-suppressing medicaments, health-care food or cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Inhibitory effect of breviscapine extract on tumor angiogenesis in vitro

[0031] Liposarcoma cells SW872 were placed in DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Incubator cultivation. Take the cells in the logarithmic growth phase, and use 4*10 4 / mL cells were planted in a 24-well plate pre-coated with a layer of fibrin, placed at 37 ° C, 5% CO 2 Incubator for 24 hours. After 24 hours, add different concentrations (1-500ug / mL) of Erigeron breviscapus extract, take the blank culture medium as the control, and take 5uM 5-Fu as the positive control. After culturing for 36 hours, carefully remove the supernatant, and stain with Wright-Gimsa. solution for staining, and then select a typical area of ​​each well to take pictures. The above experiments were repeated 3 times.

[0032] The result is as Figure 1~5 As shown, various breviscapine extracts have a good inhibitory effect on tumor cell angiogenesis in vitro, and the inhibitory e...

Embodiment 2

[0033] Example 2: Inhibitory effect of breviscapine extract on Matrigel tumor angiogenesis in vitro

[0034] Take 150uL of melted BD Matrigel, add it to a 48-well plate, tap it to make it evenly distributed, and incubate at 37°C for 1 hour to make Matrigel polymerize into a gel. Take the tumor cells SW872 and 3B11 in the logarithmic growth phase, and use 4*10 5 / mL cells were mixed with equal volumes of RPMI 1640 culture medium containing different concentrations of breviscapine extract, seeded on Matrigel at 200uL / well, and incubated at 37°C, 5%C0 2 After culturing in the incubator for several hours, stain with Wright-Gimsa staining solution, observe and take pictures under a microscope.

[0035] Such as Figure 6 As shown, the results show that the ethanol extract of breviscapine has a good inhibitory effect on the in vitro Matrigel model of tumor vascular endothelial cells, and it is dose-dependent. In the control group, the blood vessels were thick, and the tubular stru...

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Abstract

The invention discloses the application of erigeron breviscapus extract in preparing anti-tumor or angiogenesis-suppressing medicine, health-care food or cosmetics. It is the first revelation that the erigeron breviscapus extract has the activities of suppressing tumor and angiogenesis, can treat or prevent the diseases relative to the tumor and the angiogenesis and can be applied to the tumor chemotherapy and / or auxiliary chemotherapy. The angiogenesis as the target point of treating the diseases is used skillfully, and the medicinal effectiveness and the mechanism of the erigeron breviscapus are studied and clarified, so that the invention provides the scientific evidence and the important information for the new target points of developing new uses and discovering drug action and is the new theory development of traditional Chinese medicine. The erigeron breviscapus extract can be applied to the medicaments, health-care products and / or cosmetics.

Description

technical field [0001] The invention relates to a breviscapine extract for inhibiting tumor and angiogenesis and its application in the preparation of anti-tumor or anti-tumor angiogenesis drugs, health food or cosmetics. Background technique [0002] Blood vessels are necessary for the morphogenesis and function maintenance of normal tissues and organs, and are closely related to human developmental disorders and various diseases caused by various etiologies. Malignant tumors are currently the culprit of people's life and health in our country, and they are also the key scientific and technological projects of the country's "population and health". Tumor neovascularization plays a pivotal role in tumor occurrence, development, metastasis and recurrence. A series of studies have shown that as long as the tumor neovascularization is effectively inhibited or the existing tumor blood vessels are destroyed, tumor formation, metastasis and recurrence can be inhibited. In additi...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P35/00A61P9/10A61P9/00A61P27/02A61P27/04A61P17/06A61P17/10A61P17/00A61P19/02A61P29/00A23L1/29A61K8/97
Inventor 周泉生顾振纶曹志飞蒋小岗朱峰妍郭次仪
Owner 苏州中药研究所有限公司
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