Method for separating phospholipids in purified oil material

Abstract

The invention relates to a method for separating phospholipids in purified oil materials, which is characterized in that (1) soybeans, eggs, fish, oil materials with higher content of phospholipids and byproducts thereof are extracted twice by 95% of ethanol and are filtered, and the extracts are combined and steamed in a rotation mode to obtain crude fat containing the phospholipids; (2) silica gel is weighed and arranged in a chromatographic column, and the height of the silica gel is 2-5cm; (3) the crude fat sample is weighed and loaded, neutral fat and free fatty acid are eluted by ethylacetate firstly, then cephalin is eluted by absolute ethyl alcohol, and finally lecithin is eluted by 80-95% of ethanol after PE is eluted; and (4) the eluent is decompressed and concentrated to be dry. The invention separates PE, PC, neutral fat and free fatty acid in the lipid at one step via silica gel column chromatography by safe solvents, the equipment is simple, the dosage of silica is small, the reagent consumption is low, the cost is low, the application range is wide, and the oil materials containing the phospholipids can be flexibly applied according to specific circumstances.

Description

technical field [0001] The invention belongs to the field of deep processing of agricultural and forest by-products. Background technique [0002] There have been many studies and practices in my country on the extraction of phospholipids from various oil plants and their applications. Solvent extraction method, the most commonly used solvent is acetone, acetone can dissolve oil and free fatty acids but not phospholipids, so acetone can be used to remove the oil in phospholipids, and then use absolute ethanol or hydrous ethanol to extract phospholipids. In solvent extraction, the extraction times are many and the time is long, and acetone is harmful to the human body. When the acetone-treated product is used in food, there is acetone residue. supercritical fluid CO 2 Extraction method, the basic principle of which is to convert CO 2 The fluid is filled into a special pressure and temperature device to selectively extract neutral lipids from oil samples in a supercritical ...

AI Technical Summary

Problems solved by technology

The commonly used adsorbents are silica gel, alumina, diatomaceous earth, etc.; the eluent often uses organic solvents with certain toxicity such as chloroform, acetone, methanol, etc. The solvent residue in the product is the shortcoming of this method

There are also reagent safety issues in methods such as preparation of thin layer chromatography and inorganic salt composite precipitation technology.