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Membrane separation method for microalgae extracellular polysaccharide

An extracellular polysaccharide and membrane separation technology, applied in the field of microalgae biotechnology and bioseparation engineering, can solve the problems of time-consuming, labor-intensive, difficult to scale production, etc. Effect

Inactive Publication Date: 2012-06-27
WUHAN UNIV
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Problems solved by technology

[0004] At present, the separation and purification of exopolysaccharides from microalgae mostly adopt the traditional method, that is, removal of algae cells - concentration under reduced pressure - ethanol precipitation - dialysis - protein removal - freeze drying, this method is not only time-consuming and laborious but also low in efficiency, and it is difficult to scale up Production

Method used

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  • Membrane separation method for microalgae extracellular polysaccharide
  • Membrane separation method for microalgae extracellular polysaccharide
  • Membrane separation method for microalgae extracellular polysaccharide

Examples

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Effect test

Embodiment 1

[0026] Embodiment 1: Separation and preparation of Spirulina exopolysaccharide

[0027] Spirulina is the most common type of microalgae large-scale cultivation at home and abroad. A large number of studies have proved that spirulina is rich in nutrients and has positive effects in enhancing immunity and regulating body metabolism. Spirulina polysaccharide is a kind of natural active product isolated from spirulina, which has the functions of improving immunity, anti-aging, anti-tumor and promoting cell growth.

[0028] The large-scale separation and preparation steps of Spirulina exopolysaccharide in the present invention are as follows:

[0029]1. Clarification and pre-filtration: 1) Sedimentation treatment. 450L of spirulina culture solution cultivated for 30 days was naturally settled for 12 hours, and the upper layer was clarified algae-removing culture solution; 2) pre-filtering. The above-mentioned algae-removing culture solution was pre-filtered through two layers of ...

Embodiment 2

[0034] Example 2: Isolation and preparation of exopolysaccharide from Haematococcus pluvialis

[0035] 1. Clarification and pre-filtration: 1) Culture solution sedimentation treatment. 450L of Haematococcus pluvialis culture solution cultivated for 8 days was naturally settled for 12 hours, and the upper layer was clarified algal removal culture solution; (2) pre-filtered. The above-mentioned algae-removing culture solution is pre-filtered through two layers of gauze, 200-mesh mesh and 500-mesh mesh in sequence.

[0036] 2. Radial flow microfiltration to remove impurities. Adjust the feed liquid flow rate to 1 L / min at ambient temperature, and obtain the microfiltration permeate through radial flow microfiltration.

[0037] 3. Separation of exopolysaccharides from Haematococcus pluvialis by tangential flow ultrafiltration. Select an ultrafiltration membrane with a molecular weight cut-off of 5000Da, adjust the feed liquid inlet pressure to 0.9bar, and the reflux discharge p...

Embodiment 3

[0038] Example 3: Isolation and preparation of chlorella exopolysaccharide

[0039] 1. Clarification and pre-filtration: 1) Culture solution sedimentation treatment. Take 145L of chlorella culture solution that has been cultivated for 10 days, add 100mg / L alum, flocculate and settle for 12 hours, and the upper layer is clarification and algae removal culture solution; 2) pre-filter. The above-mentioned dealgae culture solution is passed through two layers of gauze, 200-mesh mesh and 500-mesh mesh successively for pre-filtration.

[0040] 2. Radial flow microfiltration to remove impurities. Adjust the feed liquid flow rate to 1 L / min at ambient temperature, and obtain the microfiltration permeate through radial flow microfiltration.

[0041] 3. Separation of chlorella exopolysaccharide by tangential flow ultrafiltration. Select an ultrafiltration membrane with a molecular weight cut-off of 5000Da, adjust the feed liquid inlet pressure to 0.9bar, and the reflux discharge pres...

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Abstract

The invention discloses a membrane separation method for microalgae extracellular polysaccharide. The method comprises the following steps of: removing suspended impurities with large particle size in algae-eliminating culture solution by using radial flow microfiltration technology; and separating the microalgae extracellular polysaccharide in the culture solution by using tangential flow microfiltration technology. The conventional microalgae extracellular polysaccharide extracting and separating technology has complex operation process and limited processing capacity, the technology of theinvention can realize scale rapid separation of the microalgae extracellular polysaccharide so as to provide biological active polysaccharide which is effectively separated from a large amount of algae-eliminating culture solution for microalgae cultivating enterprises.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology and bioseparation engineering, and in particular relates to a method for separating microalgae extracellular polysaccharides. technical background [0002] Microalgae and related products are closely related to human food and health, so the microalgae industry has achieved an unusually vigorous and strong development trend. Today's microalgae industry is prepared with an annual output of more than 10,000 tons and an output value of more than 1.3 billion US dollars. attention. my country's microalgae industrialization began in the 1950s, and industrial production began in the 1980s. With the strong support of relevant national policies and widespread attention from all walks of life, my country's microalgae industry has developed very rapidly in the past decade and has achieved remarkable results. Various microalgae breeding units and enterprises have been established and put into production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00B01D61/58
Inventor 黄泽波刘永梅李章伟李海峰李娜周双辉
Owner WUHAN UNIV
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