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Method for drying and storing activated lactobacillus and lactobacillus preparation

A lactic acid bacteria liquid, lactic acid bacteria technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve problems such as difficulties, and achieve the effect of small loss of viable bacteria, better use effect, and improved recovery rate.

Inactive Publication Date: 2010-09-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is very difficult to remove water by airflow drying in actual production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the dry preservation method of active lactic acid bacteria and the lactic acid bacteria inoculum agent that obtains

[0049] 1. The preparation method of bacterial agent (that is, the dry preservation method of active lactic acid bacteria)

[0050] (1) Drying of the carrier

[0051] The carrier is wheat flour. Select conventional non-mildew commercial barley, grind it, and pass through a 20-mesh sieve.

[0052] The wheat flour was air-dried at 120°C for 2 hours, and its moisture content was detected. The results showed that the moisture content of the dried wheat flour was 5% (mass percentage). The dried wheat flour is collected in the buffer bin, and cooled to 40°C by suction.

[0053] If the material in the buffer bin does not exceed 1 ton, the cooling time generally does not exceed 2 hours, and the moisture content of the dried wheat flour only increases by about 0.3%, which has little effect on the subsequent adsorption operation. If necessary, af...

Embodiment 2

[0071] Embodiment 2, the dry preservation method of active lactic acid and the lactic acid bacteria bacterial preparation that obtains

[0072] 1. The preparation method of bacterial agent (that is, the dry preservation method of active lactic acid bacteria)

[0073] (1) Drying of the carrier

[0074] The carrier is corn flour.

[0075] The drying method was the same as that described in Example 1, and the moisture content of the dried corn flour was 7%. The dried cornmeal was cooled to 40°C.

[0076] If necessary, after the cooling is completed, the dried corn flour can be vacuum-packed to prevent moisture absorption.

[0077] (2) Obtaining of lactic acid bacteria liquid

[0078] The lactic acid bacterium is Lactobacillus fermentium (Lactobacillus fermentium) CGMCC 1.880, which was purchased from the General Microbiology Center of China Committee for the Collection of Microorganisms.

[0079] The culture method of following Lactobacillus fermentium (Lactobacillus ferment...

Embodiment 3

[0097] Embodiment 3, the dry preservation method of active lactic acid and the lactic acid bacteria bacterial preparation that obtains

[0098] 1. The preparation method of bacterial agent (that is, the dry preservation method of active lactic acid bacteria)

[0099] (1) Drying of the carrier

[0100] The carrier is wheat bran.

[0101] The wheat bran was air-dried at 90° C. for 2 hours, and its moisture content was detected. The result showed that the moisture content of the dried wheat bran was 8%. Cool the dried wheat bran to 40°C.

[0102] If necessary, after the cooling is completed, the dried wheat bran can be vacuum-packed to prevent moisture absorption.

[0103] (2) Obtaining of lactic acid bacteria liquid

[0104] The lactic acid bacterium is Lactobacillus fermentium (Lactobacillus fermentium) CGMCC 1.880, which was purchased from the General Microbiology Center of China Committee for the Collection of Microorganisms.

[0105] Lactic acid bacteria cultivation met...

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PUM

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Abstract

The invention discloses a method for drying and storing activated lactobacillus and a lactobacillus preparation. The method for preparing the lactobacillus preparation is as follows: mixing lactobacillus thallus or lactobacillus bacterial liquid and carrier with specific moisture content to obtain mixture, i.e. the lactobacillus bactericide. The experiments prove that the method causes the bactericide to have low viable bacterial loss, stable quality, long quality guarantee period and better using effect, saves the dehydration operation step of the lactobacillus bacterial liquid and obviouslyimproves the recovery rate of the activated lactobacillus. In addition, the method has simple and convenient operation and greatly reduces the production cost. Although the concentration of the lactobacillus in the finished product is lower, for the very low production cost, the additive proportion can be bigger for the lactobacillus preparation used by livestock and poultry. As long as the carrier is feedstuff, the additive proportion does not cause difficulty for the nutrition proportion of the feed.

Description

technical field [0001] The invention relates to a method for dry preserving active lactic acid bacteria and a lactic acid bacteria preparation. Background technique [0002] Lactic acid bacteria preparation is a very effective live bacteria preparation for livestock and poultry breeding, which can improve the digestion and health of livestock and poultry. However, lactic acid bacteria are generally sensitive to heat and oxygen. Lactic acid bacteria are generally cultured in liquid, and the drying and preservation of the finished product play a decisive role in the quality of the product. [0003] With conventional air drying, the activity loss of lactic acid bacteria generally exceeds 90%. Even with instant spray drying, the loss of vigor was more than 85%. When the dried preparation is exposed to the air for 24 hours under the condition of 20° C., the loss of vitality will reach more than 40%, which has caused great difficulties for practical popularization and use. [0...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/20C12R1/225
Inventor 陆文清胡婷曹云鹤董冰李德发
Owner CHINA AGRI UNIV