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Molecular marker method for tracking Jingzhou rye chromosome 6RL powdery mildew resistance genes

A powdery mildew resistance gene and chromosome technology, applied in the field of plant biology, can solve problems such as threats to wheat production and loss of resistance, and achieve the effect of accelerating transfer and utilization

Inactive Publication Date: 2010-09-29
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, due to the evolution of the powdery mildew physiological race, the resistance of many genes including the rye Pm8 gene has been lost (see reference: Bennett F G A (1984) Resistance to powderymildew in wheat: a review of its use in agriculture and breeding programs, Plant Pathology, 33:279-300), a serious threat to wheat production

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  • Molecular marker method for tracking Jingzhou rye chromosome 6RL powdery mildew resistance genes
  • Molecular marker method for tracking Jingzhou rye chromosome 6RL powdery mildew resistance genes
  • Molecular marker method for tracking Jingzhou rye chromosome 6RL powdery mildew resistance genes

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Embodiment Construction

[0021] The following embodiments are for better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores.

[0022] All primers were synthesized by Yingjun Biotechnology Co., Ltd. (Shanghai). All wheat materials used in the following examples are known and used.

[0023] (1) Development of EST markers and their location on chromosomes of Jingzhou rye

[0024] By downloading ESTs mapped to wheat chromosome group 6 from Graingenes (http: / / www.graingenes.org) and GenBank (http: / / www.ncbi.nlm.nih.gov) (see references: Randhawa HS, Dilbirligi M, Sidhu D, et al. (2004) Deletion mapping of homoeologous group 6-specific wheat expressed sequence tags. Genetics, 168: 677-686), using the Primer Premier 5.0 program to design ...

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Abstract

The invention discloses a molecular marker method for tracking Jingzhou rye chromosome 6RL powdery mildew resistance genes, and belongs to the technical field of plant biology. The provided primer pair is any one of three pairs of EST-STS primers and comprises the primer pair of CINAU145, CINAU450 and CINAU895. The molecular marker method is characterized in that: the markers can amplify strips which are different from wheat in the Jingzhou rye, are positioned on a long arm of the 6R chromosome through the amplification analysis in Huixian red wheat and Jingzhou rye 6R addition line and telomere addition line, are favorable for rapidly identifying 6RL-carrying powdery mildew resistance genes in the wheat background, and are used for the marker-assisted selection.

Description

1. Technical field [0001] The patent discloses a molecular marker method for tracing Jingzhou rye chromosome 6RL powdery mildew resistance gene, which belongs to the field of plant biotechnology. 2. Background technology [0002] Wheat powdery mildew caused by powdery mildew (Blumeria graminis DC.) is one of the important diseases threatening wheat production. The resistance of wheat to powdery mildew is generally controlled by a single gene, which is consistent with the gene-to-gene hypothesis. There are 43 powdery mildew resistance genes officially named in the world, including nearly 60 loci (see references: McIntosh RA, Yamazak Y, Dubcovsky J, Rogers J, Morris C, SomersDJ, Appels R, Devos KM (2008 ) Catalog of gene symbols for wheat. Proc 11th Inter Wheat Genet Symp, August 24-29, Brisbane, Qld Australia; Hua W, Liu ZJ, Zhu J, Xie CJ, Yang TM, Zhou YL, Duan XY, SunQX, Liu ZY (2009) Identification and genetic mapping of Pm42, a new recessive wheat powdery mildew resista...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11A01H1/04C12R1/645
Inventor 亓增军庄丽芳王丹孙玲冯祎高刘振乾
Owner NANJING AGRICULTURAL UNIVERSITY