Method for determining polysorbate nonionic surfactant
A surfactant, polysorbate technology, applied in the field of chemical determination, can solve the problems affecting the migration and degradation of other coexisting organic substances, toxicity and so on
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Embodiment 1
[0020] Example 1 Determination of the amount of polysorbate 20 adsorbed by sand.
[0021] (1) Pass the sand through a sieve with a diameter of 2 mm, weigh 2 g of sand and place it in a 250 mL conical flask.
[0022] (2) Add accurately 2000mg·L to the conical flask -1 50mL of polysorbate 20 solution, immediately covered with a rubber stopper lined with polytetrafluoroethylene.
[0023] (3) The conical flask was shaken at a constant temperature (30°C) in a shaker at 150 rpm for 24 hours to ensure that the adsorption reached equilibrium.
[0024] (4) Take out the Erlenmeyer flask after 24h and centrifuge at 4500prm for 10min.
[0025] (5) Take the supernatant after centrifugation, measure the concentration of the polysorbate 20 solution in the solution by the method described in the present invention, and calculate the adsorption amount of the sand polysorbate 20 by the subtraction method.
[0026] (6) After determination, the concentration is 2000mg·L -1 The equilibrium concentration of po...
Embodiment 2
[0027] Example 2 Determination of the amount of polysorbate 20 adsorbed by sandy loam.
[0028] (1) Pass the sandy loam soil through a sieve with a diameter of 2 mm, and weigh 2 g of the sieved sandy loam soil into a 250 mL conical flask.
[0029] (2) Accurately add 50 mL of polysorbate 20 solution with a concentration of 1600 mg / L to the conical flask, and immediately cover with a rubber stopper lined with polytetrafluoroethylene.
[0030] (3) The conical flask was shaken at a constant temperature (30°C) in a shaker at 150 rpm for 24 hours to ensure that the adsorption reached equilibrium.
[0031] (4) Take out the Erlenmeyer flask after 24h and centrifuge at 4500prm for 10min.
[0032] (5) Take the supernatant after centrifugation, use the method described in the present invention to determine the concentration of the polysorbate 20 solution in the solution, and calculate the amount of sand adsorbed on the polysorbate 20 by the subtraction method.
[0033] (6) After determination, the ...
Embodiment 3
[0034] Example 3 Determination of the amount of clay adsorbed to polysorbate 20.
[0035] (1) Pass the sandy loam soil through a sieve with a diameter of 2 mm, and weigh 2 g of the sieved clay into a 250 mL conical flask.
[0036] (2) Accurately add 800mg L to the conical flask -1 50mL of polysorbate 20 solution, immediately covered with a rubber stopper lined with polytetrafluoroethylene.
[0037] (3) The conical flask was shaken at a constant temperature (30°C) in a shaker at 150 rpm for 24 hours to ensure that the adsorption reached equilibrium.
[0038] (4) Take out the Erlenmeyer flask after 24h and centrifuge at 4500prm for 10min.
[0039] (5) Take the supernatant after centrifugation, use the method described in the present invention to measure the concentration of the polysorbate 20 solution in the solution, and calculate the amount of clay adsorbed on the polysorbate 20 by the subtraction method.
[0040] (6) After determination, the concentration is 1600mgL -1 After being adsor...
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