Sensor for detecting penicillin in liquid

A technology of penicillin and sensors, applied in the field of biosensors, can solve problems such as prone to false positive results, high requirements for instruments, cumbersome operations, etc., and achieve the effects of improving detection sensitivity, high sensitivity, and simplifying operating procedures

Inactive Publication Date: 2010-10-13
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

Although these methods can detect antibiotic residues in milk, they also have shortcomings such as time-consuming, manpowe

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  • Sensor for detecting penicillin in liquid
  • Sensor for detecting penicillin in liquid
  • Sensor for detecting penicillin in liquid

Examples

Experimental program
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Example Embodiment

[0040] Example 1

[0041] Prepare the sensor for detecting penicillin in liquid according to the following steps:

[0042] a. Use 1.0μm, 0.3μm, 0.05μm Al in turn on the front end of the platinum disk electrode 2 O 3 The powder is polished into a mirror surface, and each step is washed with double distilled water, and then the platinum disc electrodes are placed on the HNO 3 , Ultrasonic cleaning in absolute ethanol and double distilled water for 15 minutes, the treated electrode is dried at room temperature for later use; the egg yolk lecithin and cholesterol are prepared into a BLM film-forming solution at a mass ratio of 5:2, and 5μl of BLM film-forming solution Use a micropipette to drop on the cleaned electrode surface, keep it at room temperature for 20 minutes, then insert the electrode into a 0.1 mol / L KCl solution and soak for 15 minutes to obtain a BLM-modified platinum disk electrode and a BLM-modified platinum disk The electrode is represented by Pt-BLM;

[0043] b. Inser...

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Abstract

The invention provides a sensor for detecting penicillin in liquid. A method for preparing the sensor comprises the following steps of: electroplating nanogold on a platinum disk electrode modified by a bilayer lipid membrane; and combining penicillin antibody on the platinum disk electrode modified by the nanogold bilayer lipid membrane to make the platinum disk electrode modified by the nanogold bilayer lipid membrane and combined with the penicillin antibody. The sensor has high sensitivity and an acceptable linear range and can save the time and simplify the operating procedure.

Description

technical field [0001] The invention belongs to the field of biosensors, and relates to a biosensor for detecting antibiotic residues, in particular to a nano-bilayer lipid membrane (Nano-BLM) biosensor for detecting antibiotic residues in food. Background technique [0002] Penicillin belongs to the class of β-lactam antibiotics that are frequently used in veterinary therapy to prevent bacterial infections. This leaves penicillin residues in the milk and certain amounts of penicillin can cause allergic reactions in some people. At the same time, penicillin residues will also promote the emergence of drug-resistant strains of bacteria. In order to avoid risks from residues in milk or from edible tissues obtained from treated animals, countries have established maximum residue limits (MRLs). For example, EU Regulation 508 / 1999 sets the MRL for penicillin in milk at 4 ppb or 1.2×10 -8 M. At present, the detection methods of antibiotic residues in milk are generally as foll...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 李媛媛许冰夏玮周寅徐顺清
Owner HUAZHONG UNIV OF SCI & TECH
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