Preparation method of potable spirit rich in gamma-aminobutyric acid
A technology of aminobutyric acid and beverages, applied in the preparation of alcoholic beverages, etc., can solve the problems of insufficient supplementation and scarce content, and achieve the effects of preventing obesity, promoting ethanol metabolism, and improving hyperlipidemia
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Embodiment 1
[0047] 1. Preparation of Saccharomyces cerevisiae Seed Solution
[0048] 1.1 Activation of fermentation strains
[0049] Saccharomyces cerevisiae - CICC31481, Saccharomyces cerevisiae.
[0050] Medium: 5 ° Bé wort.
[0051] Measure 10ml of 0.9% normal saline into a clean test tube, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacteria powder in the ampoule of Saccharomyces cerevisiae into 0.9% normal saline under aseptic condition Shake it to dissolve it, and activate it in a 30°C incubator for 30 minutes, and set it aside.
[0052] 1.2 Expanded cultivation of fermentation strains
[0053] 1.2.1 Preparation of primary fermentation seed liquid
[0054] Measure 100ml of 5°Bé wort into a 500ml Erlenmeyer flask, sterilize at 121°C for 20 minutes and cool to 30°C, inoculate 10% of the volume of the culture medium with activated strains, shake at 30°C and 150 rpm Bed culture for 48 hours, as a primary fermentation seed.
[0055] 1.2.2 Preparati...
Embodiment 2
[0083] 1. Preparation of Saccharomyces cerevisiae Seed Solution
[0084] 1.1 Activation of fermentation strains
[0085] Saccharomyces cerevisiae - CICC31481, Saccharomyces cerevisiae.
[0086] Medium: 5 ° Bé wort.
[0087] Measure 10ml of 0.9% normal saline into a clean test tube, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacteria powder in the ampoule of Saccharomyces cerevisiae into 0.9% normal saline under aseptic condition Shake it to dissolve it, and activate it in a 30°C incubator for 30 minutes, and set it aside.
[0088] 1.2 Expanded cultivation of fermentation strains
[0089] 1.2.1 Preparation of primary fermentation seed liquid
[0090] Measure 100ml of 5°Bé wort into a 500ml Erlenmeyer flask, sterilize at 121°C for 20 minutes and cool to 30°C, inoculate 10% of the volume of the culture medium with activated strains, shake at 30°C and 150 rpm Bed culture for 48 hours, as a primary fermentation seed.
[0091] 1.2.2 Preparati...
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