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Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether

A technology of decabromodiphenyl ether and calcium acetate, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of degrading decabromodiphenyl ether that have not been reported, and achieve no secondary pollution, low cost, high efficiency effect

Inactive Publication Date: 2010-12-01
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no strains of Acinetobacter have been reported to have the ability to degrade decabromodiphenyl ether

Method used

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  • Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether
  • Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Debromination degradation test of Acinetobacter calcoaceticus DB-3 on decabromodiphenyl ether with a concentration of 10mg / L:

[0040] Degradation basal medium formula: Each liter of medium contains 1g of peptone, 0.5g of yeast extract, KH 2 PO 4 2.93g, Na 2 HPO 4 8.87g, NH 4 Cl 1.0g, NaCl 0.5g, MgSO 4 5g, CaCl 2 2H 2 O 0.15g, the balance is water.

[0041] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0042] Add 1ml of 300mg / L decabromodiphenyl ether stock solution dissolved in dichloromethane to a 100ml conical flask, avoid light until the dichloromethane volatilizes, then add 30ml of degradation basal medium to the conical flask, shake well to obtain 30ml of degradative Experiment medium.

[0043] That is, the formula of the degradation experiment medium: each liter of medium contains 10 mg of decabromodiphenyl ether, 1 g of peptone, 0.5 g of yeast extract, and KH 2 PO 4 2.93g, Na 2 HPO 4 8.87g, NH 4 Cl 1.0g...

Embodiment 2

[0053] Debromination degradation test of Acinetobacter calcoaceticus DB-3 on decabromodiphenyl ether with a concentration of 10mg / L:

[0054] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0055] The degradation test medium in this embodiment is the same as the degradation test medium in Example 1.

[0056] 2. Cultivate the Acinetobacter calcoaceticus DB-3 pure bacteria of the present invention on LB plate, then pick a single colony from the solid LB plate and inoculate it into 30ml of degradation experiment medium, cultivate it at 30°C and 150 rpm in the dark, and cultivate it for 60h Finally, take the culture solution, utilize ion chromatography to analyze the bromide ion concentration in the culture solution, and do three parallel samples in the experiment.

[0057] 3. Determination of the degradation rate of Acinetobacter calcoaceticus DB-3:

[0058] Take 2mL of the above-mentioned culture solution from three parallel samples,...

Embodiment 3

[0060] Debromination degradation test of Acinetobacter calcoaceticus DB-3 on decabromodiphenyl ether with a concentration of 10mg / L:

[0061] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0062] The degradation test medium in this embodiment is the same as the degradation test medium in Example 1.

[0063] 2. Routinely culture the Acinetobacter calcoaceticus DB-3 pure bacteria of the present invention on an LB plate, then pick a single bacterium from the solid LB plate and inoculate it into a 30ml degradation experiment medium, and culture it at 30°C and 150 rpm in the dark for 72 hours , take the culture solution, use ion chromatography to analyze the bromide ion concentration in the culture solution, and do three parallel samples in the experiment.

[0064] 3. Determination of the degradation rate of Acinetobacter calcoaceticus DB-3:

[0065] Take 2mL of the above-mentioned culture solution from three parallel samples, filter i...

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Abstract

The invention discloses acinetobacter calcoaceticus DB-3 and application thereof in degrading decabromodiphenyl ether. The acinetobacter calcoaceticus is preserved in the China Center for Type Culture Collection (CCTCC) on November 27th, 2009, and the collection number is CCTCC No:M 209287. The acinetobacter calcoaceticus has relatively strong degradation capability for the decabromodiphenyl ether and can debrominate the decabromodiphenyl ether to produce dissociative bromonium ions. After the biological degradation of the acinetobacter calcoaceticus, the decabromodiphenyl ether does not produce highly-toxic secondary pollutants such as polybrominated dibenzo-p-dioxin, polybrominated dibenzofuran, low-brominated diphenyl ether and the like. The acinetobacter calcoaceticus strain can be used for controlling the decabromodiphenyl ether pollution in the environment so as to provide low-cost and high-efficiency decabromodiphenyl ether degrading bacteria without secondary pollution for treating the decabromodiphenyl ether in chemical industrial sludge or wastewater.

Description

Technical field: [0001] The invention belongs to the field of microorganism application, and in particular relates to Acinetobacter calcoaceticus DB-3 and its application in degrading decabromodiphenyl ether. Background technique: [0002] Decabromodiphenyl ether (BDE2209 or DeBDE) is the most demanded polybrominated diphenyl ether in the market. As a kind of brominated flame retardants (BFRs), it is widely used as impact-resistant polystyrene, polyester , polyamide, textile and electronics additives. In recent years, the demand for decabromodiphenyl ether has continued to increase. According to statistics, in 1999, the demand for BDE2209 accounted for 81% of the total demand for polybrominated diphenyl ethers in the world, and in 2001 it was 83%. As of 2001, the total output of flame retardants in my country was about 1.5×10 5 t, while the sales volume of BDE2209 has reached 1.35×10 4 t. The content of decabromodiphenyl ether in the global natural environment shows a si...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
Inventor 邓代永许玫英郭俊孙国萍陈杏娟
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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