Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether

A technology of decabromodiphenyl ether and calcium acetate, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of degrading decabromodiphenyl ether that have not been reported, and achieve no secondary pollution, low cost, high efficiency effect

Inactive Publication Date: 2010-12-01
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no strains of Acinetobacter have been repor

Method used

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  • Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether
  • Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example one:

[0039] Debromination and degradation test of Acinetobacter calcoaceticus DB-3 on Decabromodiphenyl ether at a concentration of 10mg / L:

[0040] Degradation basic medium formula: each liter of medium contains peptone 1g, yeast extract 0.5g, KH 2 PO 4 2.93g, Na 2 HPO 4 8.87g, NH 4 Cl 1.0g, NaCl 0.5g, MgSO 4 5g, CaCl 2 ·2H 2 O 0.15g, the balance is water.

[0041] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0042] Add 1ml of 300mg / L decabromodiphenyl ether storage solution dissolved in dichloromethane to a 100ml Erlenmeyer flask, protect from light and wait for the dichloromethane to evaporate, then add 30ml of degradation basal medium to the Erlenmeyer flask, and shake it to get 30ml degradation Experimental medium.

[0043] That is, the formula of the degradation experiment medium: each liter of medium contains 10mg of decabromodiphenyl ether, 1g of peptone, 0.5g of yeast extract, KH 2 PO 4 2.93g, Na 2 HPO 4 8.87g, NH...

Example Embodiment

[0052] Embodiment two:

[0053] Debromination and degradation test of Acinetobacter calcoaceticus DB-3 on Decabromodiphenyl ether at a concentration of 10mg / L:

[0054] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0055] The degradation experiment medium of this example is the same as the degradation experiment medium of Example 1.

[0056] 2. The Acinetobacter calcoaceticus DB-3 pure bacteria of the present invention were cultured on LB plate, and then a single colony was picked from the solid LB plate and inoculated into 30ml degradation experiment medium, cultivated at 30°C, 150 rpm, and darkened for 60 hours After that, take the culture solution, analyze the bromide ion concentration in the culture solution by ion chromatography, and make three parallel samples for the experiment.

[0057] 3. Determination of the degradation rate of Acinetobacter calcoaceticus DB-3:

[0058] From the three parallel samples, take 2 mL of the culture brot...

Example Embodiment

[0059] Embodiment three:

[0060] Debromination and degradation test of Acinetobacter calcoaceticus DB-3 on Decabromodiphenyl ether at a concentration of 10mg / L:

[0061] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0062] The degradation experiment medium of this example is the same as the degradation experiment medium of Example 1.

[0063] 2. The Acinetobacter calcoaceticus DB-3 pure bacteria of the present invention are routinely cultured on an LB plate, and then a single bacteria is picked from the solid LB plate and inoculated into 30ml of degradation experiment medium. After incubating for 72 hours at 30°C, 150 rpm and dark , Take the culture solution, analyze the bromide ion concentration in the culture solution by ion chromatography, and do three parallel samples in the experiment.

[0064] 3. Determination of the degradation rate of Acinetobacter calcoaceticus DB-3:

[0065] From the three parallel samples, take 2 mL of the cultur...

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Abstract

The invention discloses acinetobacter calcoaceticus DB-3 and application thereof in degrading decabromodiphenyl ether. The acinetobacter calcoaceticus is preserved in the China Center for Type Culture Collection (CCTCC) on November 27th, 2009, and the collection number is CCTCC No:M 209287. The acinetobacter calcoaceticus has relatively strong degradation capability for the decabromodiphenyl ether and can debrominate the decabromodiphenyl ether to produce dissociative bromonium ions. After the biological degradation of the acinetobacter calcoaceticus, the decabromodiphenyl ether does not produce highly-toxic secondary pollutants such as polybrominated dibenzo-p-dioxin, polybrominated dibenzofuran, low-brominated diphenyl ether and the like. The acinetobacter calcoaceticus strain can be used for controlling the decabromodiphenyl ether pollution in the environment so as to provide low-cost and high-efficiency decabromodiphenyl ether degrading bacteria without secondary pollution for treating the decabromodiphenyl ether in chemical industrial sludge or wastewater.

Description

Technical field: [0001] The invention belongs to the field of microorganism application, and in particular relates to Acinetobacter calcoaceticus DB-3 and its application in degrading decabromodiphenyl ether. Background technique: [0002] Decabromodiphenyl ether (BDE2209 or DeBDE) is the most demanded polybrominated diphenyl ether in the market. As a kind of brominated flame retardants (BFRs), it is widely used as impact-resistant polystyrene, polyester , polyamide, textile and electronics additives. In recent years, the demand for decabromodiphenyl ether has continued to increase. According to statistics, in 1999, the demand for BDE2209 accounted for 81% of the total demand for polybrominated diphenyl ethers in the world, and in 2001 it was 83%. As of 2001, the total output of flame retardants in my country was about 1.5×10 5 t, while the sales volume of BDE2209 has reached 1.35×10 4 t. The content of decabromodiphenyl ether in the global natural environment shows a si...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
Inventor 邓代永许玫英郭俊孙国萍陈杏娟
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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