Composite microbial ecological agent and application thereof
A technology of compound microecology and preparation, applied in application, animal feed, animal feed, etc., can solve the problems of affecting the host, difficult to meet the needs of treatment, limiting the wide application of microecological preparations, etc., to improve immunity and disease resistance, The effect of improving the efficiency of feed utilization and improving the quality of animal products
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Embodiment 1
[0063] Embodiment 1, the separation, screening, selection and identification of excellent bacterial strains
[0064] 1) Bacterial enrichment culture: Inoculate fresh feces and intestinal content samples of healthy animals (pigs or poultry) into BPY liquid medium, and culture and proliferate at a constant temperature of 37°C for 48 hours;
[0065] 2) Isolation and purification of Bacillus licheniformis: step 1) enrichment culture in a 100°C water bath for 5 minutes, then streak culture on a BPY medium plate, pick out each colony with characteristic morphology, streak culture and separation, Until it is a pure colony, the Gram staining microscope is used to inoculate the isolated suspected pure colony into the inclined plane of the test tube to proliferate, and then store it in a refrigerator at 4°C until use;
[0066] Colony morphology: the surface of the colony is rough, dull, opaque milky white, with irregular edges, 2-3mm in diameter, and becomes reddish-brown as the culture...
Embodiment 2、3
[0072] Stress resistance and biological performance measurement of embodiment 2, 3 excellent bacterial strains
[0073] Three strains: Bacillus licheniformis CGMCC No.2383, Enterococcus faecalis CGMCC No.2386, Saccharomyces cerevisiae CGMCC No.2388 Determination of biological properties and stress resistance:
[0074] 1) Preparation of the culture of Bacillus licheniformis CGMCC No.2383: Inoculate the slant strains stored in the refrigerator into BPY seed medium for activation, and cultivate at 37° C. and 200 rpm for 18 hours to obtain a culture with a spore rate of more than 95%;
[0075] 2) Preparation of Enterococcus faecalis CGMCC No.2386 culture: inoculate the slant bacteria stored in the refrigerator into the MRS seed medium for activation, and culture at 35°C and 100rpm for 16h to obtain the product;
[0076] 3) Preparation of Saccharomyces cerevisiae CGMCC No.2388 culture: inoculate the slant bacteria stored in the refrigerator into No. 98 seed medium for activation, a...
Embodiment 3
[0104] Embodiment 3, the preparation of Bacillus licheniformis CGMCC No.2383 bacterial powder
[0105] 1) Plate culture rejuvenation: Inoculate the Bacillus licheniformis strain on the BPY plate medium, culture at 37°C for 24 hours, rejuvenate the Bacillus licheniformis, and form a single colony, pick a single colony and inoculate it on the slant medium, Incubate at 37°C for 36 hours;
[0106] 2) Preparation of first-class seeds: transfer the Bacillus subtilis cultured in step 1) to the eggplant bottle slant medium, and cultivate them at 37° C. for 12 hours to make them in the mid-late logarithmic period to obtain first-class seeds;
[0107] Described slant medium is BPY solid medium;
[0108] 3) Preparation of secondary seeds: make bacterial suspension from the primary seeds prepared in step 2) with sterile water, and inoculate into 1.6M 3 2M of BPY seed medium 3 In the seed tank, the temperature is 37°C, the rotation speed is 200rpm, the tank pressure is 0.05Mpa, and the ...
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