Method for purifying xylo-oligosaccharide by adsorption and separation by simulated moving bed

Abstract

The invention relates to a method for purifying xylo-oligosaccharide by adsorption and separation by a simulated moving bed, which comprises the following steps: concentrating a xylo-oligosaccharide solution containing 75.63% of xylo-oligosaccharide, 25.56% of monosaccharide and 0.81% of other components into a solution of which the concentration of saccharide in mass percent is 50% for later use; soaking cation exchange resin in a hydrochloric acid water solution for 24h, washing with water to be neutral, then soaking with sodium hydroxide for 24h, and washing with water to be neutral for later use; then preparing the xylo-oligosaccharide solution into a sample solution entering the simulated moving bed, wherein the concentration of the sample solution in mass percent is 25%; taking the cation exchange resin as an adsorbent and de-ionized water as an eluent, and continuously feeding at the temperature of 70 DEG C for absorbing and eluting to obtain the xylo-oligosaccharide solution with the purity of 95% and the yield of 75%; and concentrating the xylo-oligosaccharide solution prepared in the step c, and spray drying to obtain the xylo-oligosaccharide powder. The invention adopts the cation exchange resin as the adsorbing medium, utilizes the simulated moving bed equipment for separating and purifying the xylo-oligosaccharide solution, can realize continuous production, improves the production efficiency, reduces the consumption of resin and solvents in each step, can realize continuous production, is easy for industrialized popularization, is favorable for energy saving and emission reduction, and compensates the defect of the prior art, the recovery rate is higher than 75%, and the purity is also higher than 95%.

Description

technical field The invention belongs to a separation method of xylooligosaccharides, in particular to a method for simulating moving bed adsorption, separation and purification of xylooligosaccharides using a cation exchange resin as an adsorption medium. Background technique Xylooligosaccharide (Xylooligosaccharide) is a linear oligosaccharide composed of 2 to 7 xyloses combined with β-1,4 glycosidic bonds, but mainly disaccharides and trisaccharides, xylooligosaccharides have special physiological Function, can promote the proliferation of beneficial bacteria inherent in the human intestinal tract - bifidobacteria, thereby inhibiting the growth of spoilage bacteria in the intestinal tract and reducing the formation of toxic fermentation products. Xylo-oligosaccharides cannot be degraded by saliva, gastric juice, pancreatic juice and small intestinal juice, and can all reach the colon, with a high intestinal residual rate. At the same time, because xylooligosaccharides ar...

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Problems solved by technology

Although techniques such as polyacrylamide gel column chromatography and membrane separation can achieve good purification effects, the one-time investment for industrial production is relatively large due to the high price of the gel and the difficulty in regeneration; Due to the restriction, the production cost is greatly increased, the production efficiency is low, and the energy consumption is large, so that the application in actual production is greatly restricted; activated carbon can also achieve a certain separation effect as an adsorption medium, but the degree of continuous production is not high.

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