Oil emulsion vaccine and preparation method thereof

A technology of oil emulsion and vaccine, which is applied in antiviral agents, pharmaceutical formulations, emulsion delivery, etc., can solve problems such as difficulties, and achieve the effects of reducing retention, strong ability to induce antibody production, and strong ability to induce antibody production

Active Publication Date: 2011-01-05
PU LIKE BIO ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially for inactivated vaccines, it is very difficult to prepare a vaccine that can induce sufficient immune protection over a long period of time and has good reproducibility

Method used

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  • Oil emulsion vaccine and preparation method thereof
  • Oil emulsion vaccine and preparation method thereof
  • Oil emulsion vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 method of the present invention and conventional method prepare Newcastle disease vaccine and comparative test thereof

[0038] One. The inventive method prepares Newcastle disease antigen oil emulsion vaccine

[0039] 1. Dilute the hydrophilic non-ionic surfactant Tween-80 with water to 3 times volume, then autoclave at 121°C for 30 minutes and cool to room temperature for later use; The immune antigen was mixed evenly with Tween-80 diluted with 3 times the volume of water and sterilized at 121°C for 30 minutes to form the aqueous phase.

[0040] 2. Mix white oil, propylene glycol, lecithin and isomannide monooleate as an adjuvant, then mix with Span-80 to form an oil phase, sterilize by autoclaving at 121°C for 30 minutes, and cool to room temperature for later use.

[0041] 3. According to the volume ratio of oil phase: water phase = 2:1, first pour the oil phase into the emulsification tank, the stirring speed is 11,000rpm, and the temperature is 18°C,...

Embodiment 2

[0071] Embodiment 2 method of the present invention and conventional method prepare avian influenza vaccine and comparative test thereof

[0072] One. The inventive method prepares avian influenza antigen oil emulsion vaccine

[0073] 1. Dilute the hydrophilic non-ionic surfactant Tween-80 with water to 3 times the volume, then autoclave at 121°C for 30 minutes and cool to room temperature for later use; take poultry with a hemagglutination (HA) titer of 9log2 Influenza antigen and Tween-80 diluted with 3 times the volume of water and sterilized under high pressure at 121°C for 30 minutes were evenly mixed to form the water phase.

[0074] 2. Mix white oil, propylene glycol, lecithin and isomannide monooleate as an adjuvant, then mix with Span-80 to form an oil phase, autoclave at 121°C for 30 minutes, and cool to room temperature for later use.

[0075] 3. According to the volume ratio of oil phase: water phase = 2:1, first pour the oil phase into the emulsification tank, th...

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PUM

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Abstract

The invention discloses an oil emulsion vaccine and a preparation method thereof. The oil emulsion vaccine comprises 33.3 volume percent of water phase and 6.7 percent of oil phase, wherein the water phase contains vaccine antigen and hydrophilic nonionic surfactant; and the oil phase contains mineral oil, lecithin, propylene glycol, isomannitol monooleate and oleophylic nonionic surfactant. The vaccine does not contain aluminium stearate, can keep high antibody productivity in a long time and effectively solves the problems of animal stress reaction caused by vaccine injection and vaccine residue.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a poultry oil emulsion vaccine and a preparation method thereof. Background technique [0002] my country's poultry meat products have been repeatedly sealed off. From August 1, 1996 to May 24, 2001, the European Union closed my country's customs for five years on the grounds that my country's poultry meat exports contained animal diseases, pesticides, and veterinary drug residues. In 2004, the European Union completely banned the import of poultry products from my country on the grounds of bird flu, and it was not until August 2008 that the ban on poultry products from my country was lifted. Just lifted the ban and encountered the "quota" problem. The prerequisite for solving all problems is to improve the quality of poultry products in our country. In order to reduce the occurrence of poultry diseases, and at the same time avoid the negative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K47/44A61K39/12A61K39/145A61K39/17A61K39/295A61P31/14A61P31/16A61K39/39A61K47/10A61K47/24A61K47/26
Inventor 张许科孙进忠乔荣岑
Owner PU LIKE BIO ENG
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