Preparation method and application of water-soluble carbon nano particles

A carbon nanoparticle, water-soluble technology, applied in the field of nanometers, can solve the problems of weakening the acidification function, unfavorable development and application of carbon nanoparticles, high temperature, etc., and achieve the effect of high quenching rate

Inactive Publication Date: 2011-01-05
HUNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, this method requires a higher temperature, and concentrated nitric acid may have been decomposed into NO at 100 °C 2 , which weakens its acidification function, making it difficult to obtain well-dispersed carbon nanoparticles, which is not conducive to the development and application of carbon nanoparticles

Method used

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  • Preparation method and application of water-soluble carbon nano particles
  • Preparation method and application of water-soluble carbon nano particles
  • Preparation method and application of water-soluble carbon nano particles

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the beaker is placed on the burning candle to collect the black smoke, after a period of time the inside of the beaker deposits a layer of black solid, scrape off the black solid from the surface of the beaker; these black solids are placed in a vacuum oven to dry, Remove the moisture produced due to combustion to obtain dry carbon ash, store it in a dry environment for use; weigh 4mg of dry carbon ash in a 10mL round-bottomed flask, add 2mL of concentrated nitric acid and 2mL of DMF, at 60 Heat and reflux in an oil bath at ℃ for 12 hours; after the reflux, pour the reaction solution into a 5mL centrifuge tube and centrifuge. After centrifugation, it will be divided into two layers. Mix well and then centrifuge to remove the upper liquid, repeat this 5 times (the purpose is to remove the residual nitric acid in the reaction as much as possible, so as not to interfere with subsequent applications), add 2ml of secondary water to form an acidified carbon nanop...

Embodiment 2

[0029] Embodiment 2: get Tris-HCl buffer solution (10mM Mg 2+ , 50mMK + , pH=7.4) 400 μL was added into the fluorescent cell with a volume of 800 μL, and dye Cy3 was added to the fluorescent cell so that the concentration of dye Cy3 was 60 nM; the fluorescence intensity was tested, and then 10 μL of acidified carbon nanoparticle solution (the acidified The concentrated reading of the carbon nanoparticle solution is 2 mg of acidified carbon nanoparticles per milliliter of the solution, that is, the concentration is 2 mg / ml, the same as the following), shake well, stand for 10 minutes, test and record the fluorescence intensity, and calculate the fluorescence intensity of the carbon nanoparticles. The quenching efficiency is 96.28%; if figure 2 .

Embodiment 3

[0030]Embodiment 3: get Tris-HCl buffer solution (10mM Mg2 + , 50mMK + , pH=7.4) 400 μL was added into the fluorescent cell with a volume of 800 μL, and the dye FAM was added in the fluorescent cell so that the concentration of the dye FAM was 60 nM, and the fluorescence intensity was tested; then 10 μL of acidified carbon nanoparticle solution (2 mg / ml ), shake well, place for 10min, then test and record its fluorescence intensity, calculate the quenching efficiency of carbon nanoparticles to fluorescence is 70.85%, as image 3 .

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Abstract

The invention discloses a preparation method and application of water-soluble carbon nano particles. The method comprises the steps of: collecting candle ash by adopting a method of burning candles; putting the candle ash into a round bottomed flask; adding concentrated nitric acid and DMF (Dimethyl Formamide); heating and refluxing for about 12 hours in an oil bath pot of 50 DEG C to 60 DEG C to obtain a reaction solution; pouring the reaction solution into a centrifuge tube; centrifuging to obtain black solid; removing supernatant liquid; adding secondary water; mixing evenly; centrifuging to remove the supernatant liquid; and repeating the step for five times to obtain an acidified carbon nano particle solution. The application comprises the step of: adding the acidified carbon nano particle solution to a Tris-HCl buffer solution so that dye or DNA marked with the dye in the buffer solution produces fluorescence quenching. By using the method, good water-soluble carbon nano particles are obtained by using raw materials with low price and simple preparation process. The prepared carbon nano particles can be used as a high quenching agent for fluorescence quenching of related dye.

Description

technical field [0001] The invention relates to a preparation method of carbon nanoparticles (carbon nanoparticles) in carbon nanomaterials and an application thereof with high quenching performance, belonging to the field of nanotechnology. Background technique [0002] Carbon nanoparticles (CNPs) are a new type of carbon nanomaterials that have recently emerged. Sun et al. reported luminescent carbon nanoparticles with only about 5nm in 2006 on the American Chemical Society (JACS). A structure of luminescent carbon nanoparticles, but from the Raman results, the diamond structure and the graphite structure may exist in the carbon particles at the same time. This research result has stimulated people's research on luminescent carbon nanoparticles, because this kind of luminescent carbon nanoparticles is not only non-toxic and has good biocompatibility, but also its luminous intensity can be comparable to that of silicon quantum dots. It has stable luminescence and no light ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02B82B3/00
Inventor 杨荣华李继山刘金华
Owner HUNAN UNIV
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