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Treating bacteria with electric fields

An electric field and bacteria technology, applied in the field of non-target cells, can solve the problems of different cell geometry and dielectric properties, dependence on distinguishing ability, damage to normal cells, etc.

Inactive Publication Date: 2011-01-05
NOVOCURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A disadvantage of this approach is that the discriminatory power is highly dependent on the cell type, e.g. the size difference between normal and tumor cells is only significant for certain types of cells
Another disadvantage of this approach is that the applied voltage can damage some normal cells and may not destroy all tumor cells because differences in size and membrane dielectric properties are largely statistical and practical Cell geometry and dielectric properties can vary significantly

Method used

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  • Treating bacteria with electric fields
  • Treating bacteria with electric fields
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Examples

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Effect test

example 1

[0114] To demonstrate the effectiveness of electric fields having the above properties (eg, frequencies between 50 KHz and 500 KHz) in destroying tumor cells, the electric fields were used to treat mice with malignant melanoma. Two pairs of insulated electrodes 230 were placed on a corresponding pair of malignant melanomas. Only one pair was connected to the generator 210, and an alternating electric field (TC field) at 200 KHz was applied to the tumor for 6 days. One malignant melanoma was left untreated to compare treated and untreated tumors. After 6 days of treatment, the stained malignant melanoma remained clearly visible on the untreated side of the mice, whereas no tumor was visible on the treated side of the mice. The only discernible area visible on the skin is the mark representing the insertion point of the insulated electrode 230 . The fact that the tumor on the treated side was eliminated was further demonstrated by incising and inverting the skin to expose its ...

example 2

[0175] The experiment was also carried out on two different types of bacteria, Pseudomonas aeruginosa strain PAO1 and Staphylococcus aureus strain SH1000. All strains were grown in LB medium (1.0% tryptone, 0.5% yeast extract, 1% sodium chloride). The fresh flat plate bacterial strain that broth medium is cultivated is cultivated at 37 ℃ for 15 hours in the orbital shaker flask (220RPM) of 3ml liquid medium, then is diluted to predetermined 595nm absorbance in fresh LB broth medium, obtains desired like this. CFU / ml.

[0176] Figure 32A The structure of the electrode 1610 used in the experiment is shown. Each electrode is 15mm long and 5mm high. The electrode comprises an electrical conductor 1611, the outer surface of which is coated with a thin layer of lead magnesium niobate-lead titanate (PMN-PT) ceramic insulating layer 1612, a material with a high dielectric constant (ε > 5000), Their capacitance values ​​are thus about 10 nF each. The rear of the conductor 1611 wa...

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Abstract

Cells that are in the process division are vulnerable to damage by AC electric fields that have specific frequency and field strength characteristics. The selective destruction of rapidly dividing cells can therefore be accomplished by imposing an AC electric field in a target region for extended periods of time. Some of the cells that divide while the field is applied will be damaged, but the cells that do not divide will not be harmed. This selectively damages rapidly dividing cells like bacteria, but does not harm normal cells that are not dividing. Since the vulnerability of the dividing cells is strongly related to the alignment between the long axis of the dividing cells and the lines of force of the electric field, improved results can be obtained when the field is sequentially imposed in different directions.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application 60 / 947,212, filed June 29, 2007, the entire contents of which are hereby incorporated by reference. technical field [0003] The present invention relates to the selective destruction of rapidly dividing cells in a localized area, and more particularly to the selective destruction of target cells without damage to nearby non-target cells by application of an electric field with specific characteristics in vitro or in a living patient area. Background technique [0004] All living organisms proliferate through cell division, including cell cultures, microorganisms (such as bacteria, mycoplasma, yeast, protozoa, and other single-celled organisms), fungi, algae, plant cells, and the like. The dividing cells of organisms can be destroyed or their proliferation controlled by methods based on their susceptibility to certain agents. For example, certain antibi...

Claims

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Application Information

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IPC IPC(8): A61N1/40
CPCA61N1/326A61N1/40
Inventor Y·帕尔蒂M·吉拉第
Owner NOVOCURE
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