Method for detecting quality of Chinese medicinal composition for treating proliferation of mammary gland

A technology of mammary gland hyperplasia and traditional Chinese medicine granules, which is applied in drug combinations, medical formulas, measuring devices, etc., to achieve the effects of precise drug efficacy, guaranteed quality, and strong specificity

Inactive Publication Date: 2011-01-26
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no quality testing method for related products at present

Method used

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  • Method for detecting quality of Chinese medicinal composition for treating proliferation of mammary gland
  • Method for detecting quality of Chinese medicinal composition for treating proliferation of mammary gland
  • Method for detecting quality of Chinese medicinal composition for treating proliferation of mammary gland

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Effect test

Embodiment 1

[0032] 1. Prescription: Hawthorn 1200g, Fried Malt 2000g, Mildew Spatholobus 2000g, Tongcao 660g.

[0033] 2. Preparation method: decoct the above four medicinal materials with water three times, each time for 0.5 hours, combine the decoction, filter, and concentrate the filtrate to a thick paste with a relative density of 1.10-1.12 (70°C), add an appropriate amount of dextrin, and flow Bed granulation, drying, sizing, and granulation to obtain the traditional Chinese medicine granules of the present invention.

[0034] 3. The identification and content determination methods are as follows:

[0035] 1. The identification method includes the following steps:

[0036] (1) Specificity test for the identification of Spatholobus Spatholobus

[0037] Preparation of the test solution: Take 10g of the Chinese medicine granule sample, add 50ml of water to dissolve it, add 100ml of ether for ultrasonic treatment for 30min, separate the ether layer, evaporate to dryness, add 1ml of met...

Embodiment 2

[0057] Get the Chinese medicine granule of embodiment 1, identification method comprises the steps:

[0058] (1) Identification of Spathiphyllum Spatholobus: Take 15g of sample, add 50ml of water to dissolve it, add 100ml of ether for ultrasonic treatment (power 320W, frequency 40kHz) for 20min, separate the ether layer, evaporate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test sample solution. Another formononetin reference substance, add methanol to make a solution containing 1mg per 1ml, as the reference substance solution. Test according to thin-layer chromatography (Appendix VIB of "Chinese Pharmacopoeia" in 2005), take 1 μl of the reference substance solution and 16 μl of the test solution, respectively spot on the same silica gel G thin-layer plate, and mix with cyclohexane-ethyl acetate Ester-chloroform (20:12:3) was used as the developer, developed, taken out, dried in the air, and inspected under a UV lamp at 254nm. In the chromatogr...

Embodiment 3

[0068] Get the pharmaceutical composition among the embodiment 1, identification method comprises the steps:

[0069] (1) Identification of Spathiphyllum Spatholobus: take 8g of sample, add 50ml of water to dissolve it, add 100ml of ether for ultrasonic treatment (power 320W, frequency 40kHz) for 40min, separate the ether layer, evaporate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test sample solution. Another formononetin reference substance, add methanol to make a solution containing 1mg per 1ml, as the reference substance solution. Test according to thin-layer chromatography (Appendix VIB of "Chinese Pharmacopoeia" in 2005), take 3 μl of the reference substance solution and 25 μl of the test solution, respectively spot on the same silica gel G thin-layer plate, and mix with cyclohexane-ethyl acetate Ester-chloroform (20:12:3) was used as the developer, developed, taken out, dried in the air, and inspected under a UV lamp at 254nm. In the ch...

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Abstract

The invention provides a method for detecting the quality of Chinese medicinal granules which are prepared from hawthorn fruit, stir-fired malt, suberect spatholobus stem and ricepaperplant pith and are used for treating proliferation of mammary gland. The method comprises two parts of authentication and content testing, wherein in the authentication part, a formononetin reference substance, a hawthorn fruit reference medicinal material and a citric acid reference substance are used respectively to perform authentication by thin-layer chromatography; and the content testing is determined by the content of epicatechin, and one gram of the granules contains more than or equal to 0.70 mg of the epicatechin. In the method, the formononetin reference substance, the hawthorn fruit reference medicinal material and the citric acid reference substance are selected to perform reference and the size and position of speckles are compared directly, and the method has the advantages of simple and convenient operation and intuition; and the content testing is determined by the content of the epicatechin, so the quality is better guaranteed, and the medicinal effect is more definite.

Description

technical field [0001] The invention relates to a quality detection method of Chinese patent medicines, in particular to a quality detection method of a traditional Chinese medicine composition for treating mammary gland hyperplasia. Background technique [0002] Hyperplasia of mammary glands accounts for about 70-80% of breast diseases. It is the most common breast disease in women of childbearing age, and its incidence is about 40%. breast cancer risk. Therefore, effective treatment of mammary gland hyperplasia is extremely important for maintaining the health of women, improving the quality of life and preventing breast cancer. For a long time abroad, hormones, vitamin drugs or surgery have been used to treat hyperplasia of mammary glands. Since the 1980s, tamoxifen has been used to treat mammary gland hyperplasia. It has been reported that the effective rate can reach 86% for patients with positive estrogen receptors. However, side effects such as vaginal bleeding, thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8998G01N30/90G01N30/02A61P15/14
Inventor 夏培元林彩刘松青王章阳陈勇川刘耀赵映兰
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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