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New brevibacillus brevis strain and application thereof

A technology of Bacillus brevis and strains, which is applied in the field of preparing longan microbial fresh-keeping inoculants to achieve the effect of maintaining color and taste, and reducing the rate of decay.

Active Publication Date: 2011-01-26
福建省农业科学院农业生物资源研究所
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Problems solved by technology

Therefore, the application of microorganisms to the preservation of longan fruit has great potential. Gutter reported for the first time in 1953 that Bacillus subtilis had an antagonistic effect on postharvest pathogens of citrus fruits. Registration of antagonistic organisms (Huang, 1995), but there is no application of Bacillus brevis as a microbial preservative for the production of longan

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  • New brevibacillus brevis strain and application thereof
  • New brevibacillus brevis strain and application thereof
  • New brevibacillus brevis strain and application thereof

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preparation example Construction

[0032] (4) Preparation of preservative bacterial agent: NaCl and agar powder are mixed according to the mass ratio of 10:1 and then autoclaved. The mixed solution is uniformly mixed at a ratio of 4:1 to obtain the longan microorganism fresh-keeping agent.

[0033] Among them, the components of the strain medium: beef extract 0.3%, peptone 0.5%, glucose 1%, agar powder 1.7%, sterile water preparation, pH 7.0;

[0034] Components of seed medium: starch 1%, beef extract 0.5%, peptone 0.3%, sucrose 1%, yeast powder 0.5%, CaCl 2 0.5%, prepared with sterile water, pH7.0;

[0035] Components of fermentation medium: starch 0.8%, bean cake powder 3%, peptone 0.2%, sucrose 2%, CaCl 2 0.5%, antifoaming agent 0.17%, prepared with sterile water, pH7.0.

[0036] In addition, the percentages in the above-mentioned medium components are all mass ratios.

[0037] 4. Carry out fresh-keeping test on longan microbial fresh-keeping bacteria agent:

[0038] (1) Test material

[0039] The vari...

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Abstract

The invention discloses a strain which has an antagonistic effect is obtained by separating and screening from watermelon rhizosphere soil in Yongtai County, Fujian Province. The strain is brevibacillus brevis strain FJAT-0809-GLX (Brevibacillus brevis FJAT-0809-GLX). A microbial fresh-keeping bacterium agent which can keep longan fresh is obtained by adopting the fermentation liquor of the brevibacillus brevis strain FJAT-0809-GLX. The strain has the advantages that: a new brevibacillus brevis strain FJAT-0809-GLX is provided; the microbial fresh-keeping bacterium agent which can keep the longan fresh is prepared by the adopting the brevibacillus brevis strain FJAT-0809-GLX; and the bacterium agent can reduce the rotting rate of the longan and can keep the color, the luster and the taste of fruits.

Description

【Technical field】 [0001] The invention relates to a microorganism and its application, in particular to a new strain of Bacillus brevis and its application to prepare longan microbial fresh-keeping agent. 【Background technique】 [0002] At present, the proportion of fresh-keeping and stored fruits and vegetables in my country is relatively small. Due to poor fresh-keeping treatment, the annual loss rate of fruits and vegetables is 20% to 25%, and the annual loss rate of vegetables is 30%. The annual loss of fruits and vegetables is hundreds of millions of tons. If you make good use of fresh-keeping technology, fresh-keeping materials and corresponding facilities, the increased income is quite astonishing. Relevant statistics show that if the loss rate of fruits and vegetables in my country is reduced by 3% to 5%, the country can reduce fruit loss by 2 million tons per year, reducing The loss of vegetables is more than 10 million tons; if the storage and preservation volume ac...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23B7/155C12R1/01
Inventor 车建美刘波郑雪芳林抗美唐建阳朱育菁
Owner 福建省农业科学院农业生物资源研究所
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