DNA fragment for inhibiting expression of omega secaline gene in wheat 1B/1R translocation line and application thereof

Abstract

The invention discloses a DNA fragment for inhibiting the expression of an omega secaline gene in a wheat 1B / 1R translocation line and application thereof. The DNA fragment consists of an omega secaline gene coding region partial sequence, a plant intron sequence and the inverted repeat sequence of the omega secaline gene coding region partial sequence which are connected in turn. An RNAi genetic expression vector containing the DNA fragment is transferred to the wheat 1B / 1R translocation line in a transgenic way, so that the expression of the endogenous omega secaline gene can be inhibited, the adverse effects of omega secaline on the food processing quality of the wheat 1B / 1R translocation line are relieved or eliminated and the food processing quality of the wheat 1B / 1R translocation line is improved.

Description

technical field [0001] The invention relates to a DNA fragment for inhibiting the expression of omega-rye alkali gene in wheat 1B / 1R translocation line in the field of plant genetic engineering and its application in improving the processing quality of 1B / 1R translocation line wheat. Background technique [0002] Due to the advantages of high yield and wide adaptability, the 1B / 1R translocation line of wheat occupies a large share in the high-yield wheat widely promoted in my country. However, such varieties generally have poor processing quality, mainly reflected in low gluten strength, poor kneading resistance and sticky dough, which have obvious adverse effects on the processing quality of bread and noodles (Dhaliwal et al., Cereal Sci., 1990, 12 :165-175; Liu Jianjun et al., Acta Crops Sinica, 2004, 30(2):149~153). Studies have found that the ω-ryeline gene on the 1RS chromosome arm of the wheat 1B / 1R translocation line is considered to be an important reason for the po...

AI Technical Summary

Problems solved by technology

[0003] In the quality breeding of wheat 1B / 1R translocation lines as hybrid parents, individuals with 1B / 1R translocation are often eliminated from the hybrid offspring, but this At the same time, the high-yield and wide-fit genes linked to the ω-rye base gene were also eliminated, resulting in high-quality but not high-yield, poor adaptability of the bred wheat

It is difficult to break the linkage between the ω-rye alkali gene and the high-yield and wide-fit genes in conventional wheat breeding, because they are both on the exogenous 1RS chromosome arm, and the 1RS chromosome arm is difficult to integrate with the wheat under normal conditions. 1BS chromosome arms are paired, so it is difficult to solve the problem of high yield and low quality of 1B / 1R translocation wheat by traditional breeding methods

[0004] ω-rye alkali gene is a gene family including multiple members, and the DNA sequences between different family members are highly similar (Chai et al., Cell Research, 2005, 15 (8):658-664), it is feasible to mutate individual ω-ryeline genes by mutagenesis technology, but it is difficult to mutate multiple ω-ryeline genes at the same time

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