Fluorescent-marked 16 X-STR loci multiplex amplification system and application thereof

Abstract

The invention belongs to the technical field of bioinstrumentation, in particular to a fluorescent-marked 16 X-STR loci multiplex amplification system and application thereof. The system performs multiplex amplification analysis on 16 X-STR loci, namely GATA165B12, DXS101, GATA172D05, HPRTB, DXS981, DXS8378, DXS6795, GATA31E08, DXS6809, DXS6803, DXS9902, DXS6807, DXS7423, DXS7133, DXS6810 and DXS7132; the 16 loci are independent relatively and not linked mutually; and corresponding primers are respectively marked by four fluorescent markers, namely FAM, HEX, TAMRA and ROX. Based on the multiplex amplification system, an X-STR loci multiplex amplification kit suitable for Chinese people can be prepared for parentage analysis, individual recognition, sex tests, and gene location of X-linked inheritance disease, particularly for paternity identification of sisters, blooded half-sisters and skipped generations.

Description

Technical field [0001] The present invention is a biological test technology field, which involves a chromosomal typing system and its application. It specially involves a fluorescent detection system and its applications that specialize in the type of X -chromosome STR gene site. Background technique [0002] Short Tandem Repeats (STR) is a type of microbesin DNA sequence formed by 2-7 alkaline pairs as the core unit; STR has formed the STR gene site due to the change in the number of core repetitive units.Genetic polymorphism.In the human genome, there is an STR gene site every 6-10kb, providing a rich source of high information gene sites for forensic personal identification and parent-child identification. [0003] The classification of STR and other genes has the following significant characteristics in research and practical applications: good repetitiveness, stable and reliable types of classification results, simple and fast operation; the length of the fragment is genera...

AI Technical Summary

Problems solved by technology

[0006] 1. It is difficult to add genetic markers when using this type of kit;

[0007] 2. These X-STR loci are developed based on the data of groups other than the Chinese population (mainly the white population), and some of these loci have poor gene frequency distribution in the Chinese population and low individual recognition ability;

However, the STR typing disclosed in this invention is silver staining detection. Although the cost requirements for the experimental equipment are not high, there are many manual operations and heavy workload in the experiment, and the experimental results are easily affected by the proficiency of the personnel.

The application number is 200810219413.3, and the Chinese patent with the publication number CN101413030A discloses a fluorescent multiplex amplification system covering 10 X-STR loci, but the 10 X-STR loci of the system are located in the same linkage group. Haplotype frequencies need to be calculated in practices such as weight identification, and the overall identification efficiency is reduced

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