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Efficacy test method of infectious bronchitis vaccines and application thereof

A technology for bronchitis and chicken infectivity, applied in the biological field, can solve the problems of poor controllability, error in test results, long time, etc., and achieve the effect of small batch difference, small error and strong specificity.

Inactive Publication Date: 2011-01-26
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above experiments take a long time and have poor controllability. The evaluation results are often due to the different levels of animals used, and the test results often have errors.

Method used

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  • Efficacy test method of infectious bronchitis vaccines and application thereof
  • Efficacy test method of infectious bronchitis vaccines and application thereof
  • Efficacy test method of infectious bronchitis vaccines and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Efficacy test method of chicken infectious bronchitis inactivated vaccine

[0055] 1. Vaccination

[0056] 10 3-week-old SPF chickens were inoculated with chicken infectious bronchitis live vaccine (H 120 strain) 1 part (10 3.5 EID 50 ), after 21 days, blood was collected respectively, and serum was separated, and 1 feather portion (0.5ml) was boosted with chicken infectious bronchitis inactivated vaccine (intramuscular injection) respectively, and blood was collected respectively after 28 days, and serum was separated. The HI antibody titers were measured in the two sera respectively.

[0057] 2. Chicken infectious bronchitis hemagglutination inhibition (HI) test

[0058] 1. Determination of hemagglutination value of chicken infectious bronchitis HI antigen

[0059] (1) Take 25 μl of PBS with a concentration of 0.01mol / L and a pH of 7.2, and add it to each well of the V-shaped microreaction plate;

[0060] (2) Take 25 μl chicken infectious bronchitis hemagglutina...

Embodiment 2

[0081] Parallel relationship between HI antibody titer and challenge protection rate in experimental animals immunized with chicken infectious bronchitis inactivated vaccine

[0082] With 3 batches of vaccines, every batch of 30 21-day-old SPF chickens is divided into 3 groups, 10 in every group, and each eye is inoculated with infectious bronchitis live vaccine (H 120 strain) 1 part (10 3.5 EID 50 ), raised and observed in a positive pressure isolator (10 non-immunized control groups were set up at the same time), blood was collected and separated serum was tested for HI antibody on 21 days after immunization, and each chicken was injected intramuscularly with chicken infectious bronchitis inactivated vaccine to strengthen immunization at the same time. The first group is 1 feather portion (0.5ml), the second group is 1 / 2 feather portion (0.25ml), and the third group is 1 / 3 feather portion (0.17ml). After 28 days of booster immunization, blood was collected and serum was se...

Embodiment 3

[0090] The Parallel Relationship Between HI Antibody Titer and Egg Production Efficiency of Animals Immunized with Inactivated Infectious Bronchitis Vaccine

[0091] With 3 batches of vaccines, each batch was divided into 5 groups, and each group used 30 143-day-old non-immunized chickens, and the grouping experiment was carried out according to the following method.

[0092] Group A: no immunization;

[0093] Group B: 0.8ml of inactivated vaccine was subcutaneously inoculated into each chicken at the age of 164 days;

[0094] Group C: each intranasal inoculation of chicken infectious bronchitis live vaccine (H 120 ) 1 feather portion (10 3.8 EID 50 ), after 21 days of age, blood was collected, serum was separated, HI antibody was measured to calculate the geometric mean titer of HI antibody, and then 0.8ml of inactivated vaccine was subcutaneously injected at 164 days of age;

[0095] Group D: at the age of 143 days, the chicken infectious bronchitis live vaccine (H 120 ...

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Abstract

The invention discloses an efficacy test method of infectious bronchitis inactivated vaccines, which can be applied to vaccine quality control specifically comprising challenge protection rate control and egg-laying efficacy control. Experiments prove that the invention uses the method for detecting the HI antibody titer to replace the animal challenge test, and the method has the characteristics of simpleness, convenience, rapidness, accurate results, strong repeatability, strong specificity and the like and has the significance of popularization.

Description

technical field [0001] The invention relates to a method for testing the effectiveness of an inactivated chicken infectious bronchitis vaccine and an application thereof, belonging to the field of biotechnology. Background technique [0002] Infectious bronchitis (IB) is an acute and highly contagious infectious disease of chickens caused by infectious bronchitis virus (IBV). The main clinical manifestations are cough, tracheal rales, and sneezing. , kidney disease, egg production and egg quality of laying hens decreased. Chickens of various ages, sexes and breeds are susceptible to the disease, and chicks under 6 weeks of age are more susceptible. IBV infection of chicks that have not acquired maternal antibodies can cause permanent oviduct damage and lose egg-laying ability at sexual maturity. IBV infection of laying hens leads to a decrease in egg production, irregular egg shape, deformity and low quality, and a decrease in fertilization rate. IB also reduces the weight...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531G01N33/543
Inventor 张许科孙进忠乔荣岑
Owner PU LIKE BIO ENG
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