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Micro-fluid control liquid drop generation system based on liquid drop sequence assembly technology and use method

An assembly technology and droplet generation technology, applied in the field of microfluidic droplet generation system, can solve the problems of expensive processing equipment, increase the difficulty of microfluidic manipulation, complex micromachining technology, etc., and achieve the effect of simple and convenient construction

Inactive Publication Date: 2011-01-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires manual preparation of the droplet loader, which is time-consuming and laborious, and requires the interface between the droplet loader and the chip to transfer the droplet into the chip.
In addition, under the pressure drive, especially in the microfluidic system using air bubbles to space the liquid section, and the mixing ratio of screening reagents and substrate reactants needs to be strictly controlled, which increases the difficulty of microfluidic manipulation.
In addition, the above two methods are based on microfabricated chips, which require complex microfabrication techniques and expensive processing equipment, which limits their wide application in routine laboratories.

Method used

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  • Micro-fluid control liquid drop generation system based on liquid drop sequence assembly technology and use method
  • Micro-fluid control liquid drop generation system based on liquid drop sequence assembly technology and use method
  • Micro-fluid control liquid drop generation system based on liquid drop sequence assembly technology and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] figure 2It is a schematic diagram of the device of the automatic droplet generation system based on the notched tube array in Example 1. The system consists of three parts: a sample presentation device based on a notched tube array, a quartz capillary, and a microsyringe pump. The sample providing device based on the notched tube array is composed of a gear set 9 composed of two gears, one big and one small, and the small gear is controlled by a stepping motor to drive the big gear to rotate. On the large gear, 6 notched tubes 10 are equidistantly arranged; on the small gear, 3 notched tubes 10 are fixed according to an isosceles triangle. Liquid A4, liquid B5, liquid C6, liquid D11, liquid E12, liquid F13, liquid G14, liquid H15 and oil 2 required for generating droplet arrays are contained in the notched tubes respectively. When generating liquid droplets, the gear set 9 is rotated so that the required liquid in the notched tube 10 contacts the liquid inlet 3 of th...

Embodiment 2

[0048] image 3 It is a schematic diagram of the device of the automatic droplet generation system based on the porous plate in Example 2. The system consists of three parts: a sample presentation device based on a porous plate 16, a quartz capillary, and a micro-injection pump. The sample presentation device based on the porous plate 16 is composed of the porous plate 16 for containing various liquids and a three-dimensional translation stage 17 . When generating liquid droplets, move the three-dimensional translation stage 17, so that the different liquids contained in the porous plate 16 contact the liquid inlet 3 of the capillary 1 in sequence, absorb a certain volume of liquid into the capillary 1, and assemble and generate liquid droplets 7. In the example: the inner diameter of the capillary 1 is 0.5 micron, and the flow rate of the liquid driven by the driving device is 1 picoliter / minute.

[0049] Figure 4 It is a schematic diagram of the operation of dropping dro...

Embodiment 3

[0052] The oil-immersed droplet array plate 8 is made of glass, and the small pit containing the liquid droplet 7 on the droplet array plate 8 is processed by photolithography and chemical etching. The volume of the small pit is 20 nanoliters, and the capillary 1 The inner diameter is 150 microns, the flow rate of the liquid driven by the driving device is 10 nanoliters / minute, and the rest is the same as in Embodiment 2.

[0053] The volume of the wells can also be 1 nanoliter or 1 microliter, ranging from 1 picoliter to 100 microliters.

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Abstract

The invention relates to the field of liquid drop analysis, in particular to a micro-fluid control liquid drop generation system based on a liquid drop sequence assembly technology. In the invention, the micro-fluid control liquid drop generation system based on the liquid drop sequence assembly technology comprises an automatic liquid presenting device, a liquid drive device, a capillary and a liquid drop array plate, wherein the automatic liquid presenting device is connected with the liquid drop array plate, and the liquid drive device is connected with the capillary. The invention has the advantages that: (1) the construction of the system is simple and convenient without the complicated micro-processing technology and expensive processing equipment, so that the micro-fluid control liquid drop generation system is beneficial to wide application in a conventional laboratory; (2) the category and the mixture proportion of the liquid in the liquid drop can be optionally changed to generate liquid drops with different compositions; and (3) the generation of high flux automation of the liquid drops with the different compositions can be realized.

Description

technical field [0001] The field of the present invention is the field of droplet analysis, and more specifically, it relates to a microfluidic droplet generation system based on droplet sequential assembly technology and its application method. Background technique [0002] In recent years, microfluidic droplet analysis technology has received more and more attention. The droplet-based microfluidic analysis system uses immiscible liquid or gas phases as intervals between nanoliter to picoliter-sized droplets to form an independent reaction vessel, which has the advantages of eliminating diffusion, accelerating mixing, and automatic operation. With the advantages of fast analysis speed and low consumption of sample reagents, it is very suitable for high-throughput screening analysis, and has been successfully applied in the fields of chemistry, physics, biology and medicine. [0003] However, so far, most droplet analysis systems use the instability of the oil-water two-pha...

Claims

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Application Information

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IPC IPC(8): G01N35/00
Inventor 方群杜文斌孙蒙
Owner ZHEJIANG UNIV
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