Method for producing medicinal fungi polysaccharide with high anti-tumor activity

Abstract

The invention relates to a method for producing medicinal fungi polysaccharide with high anti-tumor activity, which comprises the following steps: adding pectinase or cellulose or acid protease into dry powder of mycelium according to a weight ratio of 1 to 5, and adding distilled water according to a weight ratio of 1:10; homogenizing and performing enzymolysis at 45 to 50 DEG C with pH value of 3.0 to 5.0 for 2h; after the enzymolysis, directly putting the enzymolysis mixture in 100 DEG C water bath, extracting for 2 h and centrifuging at a speed of 5,000 rpm for 20 min; collecting the supernatant, adding alcohol the volume of which is 3 times of that of the supernatant; precipitating at 4 DEG C for 12 h and centrifuging at a speed of 5,000 rpm for 20 min; and collecting the precipitate which is the polysaccharide. Compared with other enzyme methods and the conventional hot water method, the invention improves the polysaccharide yield and the anti-tumor activity of polysaccharide preparation; and compared with the conventional hot water method, the invention obviously shortens the operation time and reduces the energy consumption. The invention is a simple, high-yield and low-consumption method for preparing high anti-tumor activity fungi polysaccharide.

Description

technical field [0001] The invention relates to the fields of biopharmaceuticals and enzyme engineering, in particular to a method for producing medicinal fungi with high anti-tumor activity by using three different enzymes such as pectinase, cellulase and acid protease. Background technique [0002] Studies at home and abroad have proved that polysaccharides of fruiting bodies and mycelia of medicinal fungi have significant anti-tumor and immunoregulatory activities. After extracting for 1-2 hours, centrifuge to obtain the supernatant, and then continue to extract the precipitate in a boiling water bath for 1-2 hours, then centrifuge. After repeated extraction for 3-5 times, combine the supernatant and add 3 times Precipitate with volume ethanol for 24 hours, and collect the precipitate after centrifugation, which is polysaccharide. It can be seen that this method not only has complicated operation steps, high energy consumption, and long time consumption, but also the yiel...

AI Technical Summary

Problems solved by technology

[0003] Although the enzymatic method can significantly increase the yield of fungal polysaccharides, there are few reports on the antitumor activity of polysaccharides produced by enzymatic methods. So far, only one case has been reported on the antitumor activity of polysaccharides obtained by enzymatic hydrolysis, but there is no comparison of polysaccharides obtained by enzymatic hydrolysis. The anti-tumor activity of the polysaccharide extracted by the conventional hot water method is different, so it is impossible to judge the anti-tumor activity of the polysaccharide obtained by enzymatic hydrolysis

Our comparative study on 9 kinds of enzymes shows that, compared with the hot water extraction method, different enzymes not only have a significant impact on the extraction rate of mycelia polysaccharides, but also have a significant impact on the antitumor activity of the extracted polysaccharides, some Although the enzyme can significantly increase the yield of polysaccharides, it also leads to a significant decrease in the anti-tumor activity of the obtained polysaccharides, which is obviously not conducive to the production of polysaccharides with anti-tumor activity by enzymatic methods, and will lead to a decrease in the efficacy of polysaccharides prepared by enzymatic methods. disease treatment