Method for cultivating chlorella
A culturing method and technology of chlorella, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as difficult to obtain, self-cultivation of algae species, limited use scope, etc., and achieve a wide range of , the effect of increased growth rate and increased oil content
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Embodiment 1
[0034] In the process of cultivating chlorella with fish pond wastewater as a medium, the effects of temperature and light on the growth of chlorella cells were investigated. The growth condition optimization process is as follows: select the temperature of 10°C, 15°C, 20°C, 25°C, 30°C, 35°C and the light conditions of 1500Lux, 3000Lux, 4500Lux, 6000Lux, 8000Lux, 10000Lux, and carry out the combined culture of the two conditions. At different times of cultivation, the growth of Chlorella was measured. The optimization results show that the chlorella in the sewage grows well under the temperature of 20°C~30°C and the light intensity of 3000Lux~8000Lux, and the specific growth rate is above 0.1641. Among them, the temperature is 25°C and the light intensity is 4500 Lux, the growth is the best, and the specific growth rate can reach 0.1908.
Embodiment 2
[0036] Addition of different masses of Na to primary treated domestic sewage 2 CO 3, and the culture solution with the inorganic carbon concentration of 0~24mg / L was prepared respectively, and the inoculum amount of Chlorella was 10%. It was cultured for 10 days under the conditions of a light-dark ratio of 12h:12h, a temperature of 25°C, and a light intensity of 4500 Lux, and the growth of the Chlorella was measured at different times of culture. After the culture was completed, centrifuge at 4000 r / min for 10 min to harvest the cells of Chlorella algae. Lipid content and hydrocarbon content were determined after freeze-drying and preparation of algal flour.
[0037] The results show that when the concentration of inorganic carbon in the sewage is 6~18mg / L, Chlorella grows well and has a high production capacity effect, the specific growth rate can reach up to 0.2306, the oil content can reach up to 36.27%, and the hydrocarbon content can reach up to 6.67%;
Embodiment 3
[0039] Addition of different qualities of KNO to eutrophic lake water 3 , and the culture solution with a nitrogen concentration of 10-35 mg / L was prepared respectively, and the inoculum amount of Chlorella was 10%. The chlorella was cultured for 10 days under the condition that the light-dark ratio was 12h:12h, the temperature was 25°C, and the light intensity was 4500 Lux, and the growth of the Chlorella was measured at different times of culture. After the culture was completed, centrifuge at 4000 r / min for 10 min to harvest the cells of Chlorella algae. Lipid content and hydrocarbon content were determined after freeze-drying and preparation of algal flour.
[0040] The results show that when the total nitrogen concentration in sewage is 15~25mg / L, Chlorella grows well and has a high productivity effect, the specific growth rate can reach up to 0.2715, the oil content can reach up to 40.17%, and the hydrocarbon content can reach up to 11.13% %;
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