Method for fast detecting enterotoxigenic Escherichia coli K88

Abstract

The invention provides a method for fast detecting enterotoxigenic Escherichia coli K88. In the method, a fluorophore-labeled group (FAM) aptamer is selected to combine with the enterotoxigenic Escherichia coli K88, then a fluorescent signal on the aptamer is captured; an enterotoxigenic Escherichia coli K88 system to be detected is prepared, wherein the concentration of the enterotoxigenic Escherichia coli K88 is given, a fluorescence value with emission wavelength nm is detected at 455nm of excitation wavelength, and a standard curve is drawn; and a sample system to be detected is prepared,and the standard curve is checked in accordance with a fluorescence value thereof to solve the enterotoxigenic Escherichia coli K88 content in the sample to be detected. The method of the invention has the advantages of high sensitivity, strong specificity, wide measurement range, simple devices, and convenient operation, is suitable for detecting the enterotoxigenic Escherichia coli K88 content in various samples containing the enterotoxigenic Escherichia coli K88, and having very important application value in environmental monitoring, food analysis and other aspects.

Description

technical field [0001] The invention relates to a rapid detection method for Escherichia coli K88, in particular to a method for rapidly detecting Escherichia coli K88 by using the aptamer of the Escherichia coli K88 pilus protein. Background technique [0002] Environment, food carrying pathogenic bacteria is one of the most common problems in the world today. [0003] Under conditions of poor sanitation, enterotoxigenic Escherichia coli ) are often scattered in the surrounding environment with feces. If E. coli is detected in water and food, it can be considered as an indicator of feces contamination, thus proving that there may be enteropathogenic bacteria. Therefore, the number of coliform bacteria or the value of coliform bacteria is often used as the hygienic standard of drinking water and food or medicine. [0004] Escherichia coli is a resident bacterium in the intestinal tract of humans and animals. Generally, most of them are not pathogenic, but they can cause ex...

AI Technical Summary

Problems solved by technology

But the biggest disadvantage is that it is time-consuming and labor-intensive

Selective technology obviously overcomes the shortcomings of traditional methods, but the enrichment process is complicated and requires skilled workers to operate, and because a high number of target bacteria is required to obtain a positive result, the result cannot be obtained within one day

In conclusion, there is no ideal technique for detecting E. coli in the environment

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