Serum free culture medium for growing various cells derived from kidney tissue

A serum-free culture medium and culture medium technology, applied in the field of cell engineering, can solve the problems of high price, legacy problems, and unsuitability for other cell lines, and achieve the effects of low price, simple preparation method, and improved biological safety
CN101974481AInactive Publication Date: 2011-02-16国家兽用生物制品工程技术研究中心
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
国家兽用生物制品工程技术研究中心
Publication Date
2011-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a serum free culture medium for growing various cells derived from the kidney tissue. In the serum free culture medium, dulbecco's modified eagle medium (DMEM) / nutrient mixture F-12(F12)(1:1) is used as a basic culture medium and comprises the components including yeast autolysate, soy protein hydrolysate, amino acids, a fatty acid combining agent, an antioxidant reagent, reduced glutathione, a polyamine mixture, ethanolamine, beta-mercaptoethanol, glycerol, minerals, Pluronic F-68 and the like, and the pH value is 7.2. The culture medium of the invention can be used for culturing various cell lines derived from the kidney tissue, such as HEK293 cells, Marc145 cells, Vero cells and BHK21 cells and for propagating viruses.
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Description

technical field

[0001] The invention relates to a serum-free medium used for the growth of various kidney tissue-derived cells, especially capable of supporting the cultivation of HEK293 cells, Vero cells, Marc145 cells or BHK21 cells, and belongs to the field of cell engineering. Background technique

[0002] The host cells currently used for the amplification of recombinant viral vectors, recombinant protein expression or large-scale preparation of vaccines are basically mammalian kidney tissue-derived cells, such as human embryonic kidney cells (HEK293), African green ape kidney cells (Vero), Rhesus monkey kidney cells (Marc145) and young hamster kidney cells (BHK21), etc. These cells usually need to add a certain amount (5-10%) of bovine serum in the traditional culture process. The serum contains growth factors, hormones, carrier proteins, adhesion factors, trace elements and other nutrients required for cell growth, which can Promotes cell growth. However, in large-s...

Claims

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