Method for producing ethanol by fermenting raw thick mash at low temperature

A technology for concentrated mash fermentation and ethanol, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of time-consuming and energy-consuming, reduce production costs, etc. Guaranteed rapid growth

Inactive Publication Date: 2012-08-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention overcomes the time-consuming and energy-consuming shortcomings of raw material pretreatment liquefaction, cooking and saccharification in the prior art, and provides a method for producing ethanol by fermenting low-temperature raw meal thick mash. reduce manufacturing cost

Method used

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  • Method for producing ethanol by fermenting raw thick mash at low temperature
  • Method for producing ethanol by fermenting raw thick mash at low temperature

Examples

Experimental program
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Effect test

Embodiment 1

[0023] By process route figure 1 As shown, the low-temperature raw material thick mash fermentation to produce ethanol, the specific steps are as follows:

[0024] 1. Using dried cassava slices as raw materials, the dried cassava slices are crushed and calculated by weight with process water. 1 part of dried cassava slices is mixed with 1.7 parts of process water to obtain a slurry with a mass concentration of 33.3%;

[0025] 2. Heat the above-mentioned slurry to 80°C, calculate according to the weight of the dried cassava slices, add 50 units of amylase per gram of dried cassava slices to carry out low-temperature liquefaction treatment, wherein the liquefaction temperature is 80°C and the time is 45 minutes;

[0026] 3. Cool the mash after the above liquefaction treatment to normal temperature (28℃), calculate according to the weight of dried cassava slices, add 100 units of glucoamylase per gram of dried cassava slices, and access to brewing yeast (Saccharomyces cerevisiae, No. 2)...

Embodiment 2

[0034] By process route figure 1 As shown, the low-temperature raw material thick mash fermentation to produce ethanol, the specific steps are as follows:

[0035] 1. Using fresh cassava as the raw material, the cassava is crushed and calculated by weight with process water. 1 part of fresh cassava is mixed with 1.7 parts of process water to obtain a slurry with a mass concentration of 30%;

[0036] 2. Heat the above-mentioned slurry to 75°C, calculate according to the weight of the dried cassava slices, add 30 units of amylase per gram of dried cassava slices to carry out low-temperature liquefaction treatment, where the liquefaction temperature is 75°C and the time is 60 minutes;

[0037] 3. Cool the mash after the above liquefaction treatment to normal temperature (30℃), calculate according to the weight of dried cassava slices, add 80 units of glucoamylase per gram of dried cassava slices, and access to brewing yeast (Saccharomyces cerevisiae, No. 2) , Purchased from an alcohol ...

Embodiment 3

[0045] By process route figure 1 As shown, the low-temperature raw meal concentrated mash fermentation to produce ethanol, the specific steps are as follows:

[0046] 1. Using fresh cassava as the raw material, the cassava is crushed and calculated by weight with process water. 1 part of fresh cassava is mixed with 1.7 parts of process water to obtain a slurry with a mass concentration of 30%;

[0047] 2. Heat the above-mentioned slurry to 70°C, calculate according to the weight of the dried cassava slices, add 30 units of amylase per gram of dried cassava slices to carry out low-temperature liquefaction treatment, where the liquefaction temperature is 70°C and the time is 30 minutes;

[0048] 3. Cool the mash after the above liquefaction treatment to normal temperature (32°C), calculate according to the weight of dried cassava slices, add 120 units of glucoamylase per gram of dried cassava slices, and access to brewing yeast (Saccharomyces cerevisiae, No. 2) , Purchased from an alc...

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Abstract

The invention discloses a method for producing ethanol by fermenting raw thick mash at a low temperature. The method comprises the following steps of: (1) preparing over 30 mass percent powder syrup; (2) adding 30 to 50 unit / gram of amylase into the powder syrup to liquefy the powder syrup at a low temperature; (3) cooling the liquefied powder syrup to normal temperature and adding yeast and saccharifying enzyme for saccharifying and fermenting the raw thick mash synchronously, measuring the yeast concentration once every 2 hours 6 to 10 hours after fermentation, adjusting the feeding speed of the saccharifying enzyme according to the concentration change of yeast cells, entering a post maturation stage after primary fermentation is performed for 32 to 36 hours, stopping feeding the saccharifying enzyme so as to obtain mature mash 32 to 36 hours later, wherein the concentration of initial yeast is 50,000,000 / milliliter, the initial adding amount of the saccharifying enzyme is 80 to 120 units into every gram of raw material and the saccharifying enzyme is fed at the initial speed value of 5.5 units / gram of raw material every hour; and (4) rectifying the mature mash so as to obtain the ethanol. The method is simple and convenient and has low material consumption, low energy consumption and low production cost.

Description

Technical field [0001] The patent of the invention relates to a method for producing ethanol, in particular to a method for producing ethanol through low-temperature raw meal concentrated mash fermentation. Background technique [0002] Ethanol is one of the most important renewable energy products to solve the energy crisis. How to further improve its production technology, reduce its production cost, and reduce environmental pollution has always been the focus and focus of the world's energy industry. Many countries in the world have begun to use biomass resources for the industrial production of fuel ethanol. In 2008, Brazil, the largest producer of fuel ethanol, produced 30 billion liters of fuel ethanol; the EU consumed 1.75 million tons of fuel ethanol; the United States produced 11 billion gallons. The New Energy Act proposed that fuel ethanol or biofuels should be used by 2012 It will reach 23 million tons. [0003] Under the current technical conditions, the most importa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12P19/14C12R1/865
CPCY02E50/17Y02E50/10
Inventor 严宗诚陈砺王红林包莹玲
Owner SOUTH CHINA UNIV OF TECH
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