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A method of purifying a peptide

A technology for cyclic peptides and purity, applied in the field of purified peptides

Inactive Publication Date: 2011-02-23
BIOCON LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite improvements in peptide purification, some purified peptides still contain unacceptable counterions in undesired amounts

Method used

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  • A method of purifying a peptide
  • A method of purifying a peptide
  • A method of purifying a peptide

Examples

Experimental program
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Embodiment 1

[0113] A 66% pure eptifibatide TFA salt prepared by solid-phase synthesis was used for purification on a polymer-based resin-packed column. Eptifibatide TFA salt was initially dissolved in a 1:1 mixture of acetonitrile and 50 mM acetic acid to obtain a clear solution. The obtained solution was further diluted to an acetonitrile concentration of 5% and an eptifibatide concentration of <2 g / L using 50 mM acetic acid. The solution was filtered and loaded onto the column.

[0114] Amberchrom HPR10 (particle size 10 μm and pore size ) resin-packed column. The filtered eptifibatide solution was loaded onto the column at a flow rate < 360 cm / h. Loading of the peptide on the column was done at <10 g / L concentration of resin. After loading, the column was rinsed with a lower percentage (5%) of acetonitrile in 50 mM acetic acid solution. Pure product was eluted from the column by performing a linear gradient of 8-14% of acetonitrile (buffer B) for 25 CV, while 50 mM acetic acid wa...

Embodiment 2

[0120] A 69.5% pure eptifibatide TFA salt prepared by solid phase synthesis was used for purification on a polymer-based resin packed column. Eptifibatide TFA salt was initially dissolved in a 1:1 mixture of acetonitrile and 50 mM acetic acid to obtain a clear solution. The obtained solution was further diluted to an acetonitrile concentration of 5% and an eptifibatide concentration of <2 g / L using 50 mM acetic acid. The solution was filtered and loaded onto the column. The pH of sodium acetate was adjusted to 3.0 with acetic acid.

[0121] Amberchrom HPR10 (particle size 10 μm and pore size ) resin-packed column. The filtered solution of eptifibatide was loaded onto the column at a flow rate < 360 cm / h. Loading of the peptide on the column was performed at a concentration < 10 g / L of the resin. After loading, the column was rinsed with a lower percentage (5%) of acetonitrile in 10 mM sodium acetate, pH 3.0. Pure product was eluted from the column by performing a 9-12% ...

Embodiment 3

[0125] A 69.5% pure eptifibatide TFA salt prepared by solid phase synthesis was used for purification on a polymer-based resin packed column. Eptifibatide TFA salt was initially dissolved in a 1:1 mixture of acetonitrile and 50 mM acetic acid to obtain a clear solution. The obtained solution was further diluted to an acetonitrile concentration of 5% and an eptifibatide concentration of <2 g / L using 50 mM acetic acid. The solution was filtered and loaded onto the column.

[0126] Amberchrom HPR10 (particle size 10 μm and pore size ) resin-packed column. The filtered eptifibatide solution was loaded onto the column at a flow rate < 360 cm / h. Loading of the peptide on the column was performed at a concentration < 10 g / L of the resin. After loading, the column was rinsed with a lower percentage (5%) of acetonitrile in 10 mM citric acid, pH 2.5. Pure product was eluted from the column by performing a 9-12% linear gradient of acetonitrile (buffer B) for 25CV, while 10 mM citri...

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Abstract

The invention relates, interalia, to the field of purification of peptides, notably cyclic or non-cyclic peptides their analogs or derivatives thereof. More particularly, the invention relates to a simplified and optimized purification process of cyclic peptides from a composition comprising the said peptide and at least one related impurity by chromatographic procedures enabling high yields, selectivity and purity of the desired end product. The improved process is particularly useful for the preparation of eptifibatide, exenatide, atosiban, nesiritide and their respective derivatives and analogs. The polypeptides are prepared in high purity of at least about 96 %, and preferably at least about 99 %.

Description

technical field [0001] The present invention relates in particular to the field of purification of peptides, in particular cyclic or acyclic peptides, their analogs or derivatives thereof. More specifically, the present invention relates to a simplified and optimized method of purification of cyclic peptides from a composition comprising said peptide and at least one related impurity by means of a chromatographic procedure (chromatographic process) which achieves the desired High yield, selectivity and purity of the final product. The improved method is especially useful for the preparation of eptifibatide, exenatide, atosiban, nesiritide and their respective derivatives and analogs. The polypeptide is prepared at a high purity of at least about 96%, and preferably at least about 99%. Background technique [0002] An important aspect of the production of recombinant (usually genetically engineered) polypeptides, including cyclic peptides, their derivatives and their analo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00B01D15/36C07K1/18C07K1/20B01D15/32
CPCB01D15/362B01D15/325B01D15/34C07K1/36C07K7/06B01J20/285C07K14/57563C07K1/20C07K14/605C07K14/58C07K1/18B01D15/166
Inventor 尼特斯·戴夫克里希纳穆尔蒂·文卡特森拉姆普瑞布·纳贾拉雅哈里什·耶尔
Owner BIOCON LTD
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