Compound with antibacterial activity and antitumor activity and preparation method and applications thereof
A technology of anti-tumor drugs and compounds, applied in the field of microorganisms, can solve problems such as unoptimistic curative effects
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Embodiment 1
[0027] Example 1: Bacteriological properties of strain SIIA-H8968
[0028] The inventors isolated many microbial strains from the soil in Sichuan Province, my country, and conducted a series of studies on the metabolites of these strains, and found that the secondary metabolites of strain SIIA-H8968 had obvious antibacterial and antitumor activities.
[0029] The taxonomic characteristics of strain SIIA-H8968 are as follows:
[0030] 1. Morphological properties
[0031] The basal hyphae developed well, with irregular branches, but no partitions were seen. There are few aerial hyphae that form spores, and the front end of the spore chain is cord-shaped or densely helical (10-20 spores) with 1-3 turns. Spore size: 0.6-0.7 μm wide, 1.0-0.5 μm long, cylindrical, smooth surface. No sporulation was observed in the agar medium. Also, the formation of sporangia and sclerotia and the motility of spores were not observed.
[0032] 2. The nature of cultivation
[0033] Table 1 show...
Embodiment 2
[0048] Embodiment 2: the preparation of compound WSS2277
[0049] 1. The liquid containing 1.0% glucose, 2.5% soluble starch, 0.3% cottonseed meal, 0.5% fish meal, 0.3% NZ CASE (purchased from Japan Wako Pure Pharmaceutical Co., Ltd.), 0.2% yeast extract and 0.2% calcium carbonate The incubation was based on moist heat sterilization at 121°C for 20 minutes. Then inoculate an appropriate amount of strain CGMCC No.3629 under aseptic operation, shake culture at 28° C. and 200 rpm for 4 days, and use it as a seed culture solution.
[0050] Then will contain 2.0% glycerol, 2.0% dextrin, 1.0% soytone, 0.2% (NH 4 ) 2 SO 4 30 L of liquid culture medium composed of 0.2% calcium carbonate was adjusted to pH 7.0, then loaded into a 50 L fermenter, and steam sterilized at 121° C. for 20 minutes. Then, under aseptic operation, the aforementioned seed culture solution was added thereto at a 4% inoculum amount, and cultured at 28° C. and 200 rpm for 4 days.
[0051] After the cultivatio...
Embodiment 3
[0055] Embodiment 3: identification and analysis of the compound of the present invention
[0056] The chemical structure of the compound WSS2277 obtained by the above method was analyzed and confirmed by ultraviolet absorption spectrum, 1H-NMR nuclear magnetic resonance spectrum, 13C-NMR nuclear magnetic resonance spectrum and the like.
[0057] The physical and chemical properties of compound WSS2277 are as follows:
[0058] 1. Appearance: yellow amorphous powder
[0059] 2. Molecular weight: 1092
[0060] 3. Molecular formula: C 60 h 84 o 18 , the structural formula is as follows:
[0061]
[0062] 4. Ultraviolet absorption spectrum λMAX (nm): 360nm
[0063] 5.1H-NMR nuclear magnetic resonance spectrum: the sample is dissolved in deuterated dimethyl sulfoxide (DMSO-d6), and measured at 500MHz, the results are shown in figure 1 , the specific data is:
[0064] chemical shift
[0065] 6.25
[0066] Description: s singlet, d doublet, dd doublet ...
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