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Application of T-2 toxin to preparing drugs for treating pancreatic carcinoma

A technology for T-2 and pancreatic cancer, applied in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problem that the research on T-2 toxin has not yet been found.

Active Publication Date: 2011-03-16
SHENZHEN ICARBONX INTELLIGENT PEPTIDE PHARM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on T-2 toxin mainly focuses on its toxicity research and pathogenicity research on animals, and there is no report on the research on T-2 toxin used in the treatment of solid tumors.

Method used

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  • Application of T-2 toxin to preparing drugs for treating pancreatic carcinoma
  • Application of T-2 toxin to preparing drugs for treating pancreatic carcinoma
  • Application of T-2 toxin to preparing drugs for treating pancreatic carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Experimental study on the therapeutic effect of T-2 toxin on pancreatic cancer

[0015] The human pancreatic cancer tissue fragments with a diameter of 2-3mm were subcutaneously transplanted into mice. After the tumor grew to 6-7mm, the T-2 toxin dissolved in a mixture of ethanol and physiological saline was injected into the tumor at a dose of 2.5 mg / kg body weight Injection, the control group was injected with saline. The tumor size was measured with a vernier caliper or standard template on the 1, 5, 9, 14, 19, and 24 days after the injection, and the measurement lasted for 24 days. After 24 days of treatment, the tumor volume of animals in the T-2 toxin group was only 52.19% of the control group.

Embodiment 2

[0016] Example 2: Experimental study on the therapeutic effect of T-2 toxin on lung cancer

[0017] Place mouse Lewis lung cancer cells in a complete culture medium containing 10% fetal bovine serum and DMEM at 37°C, 5% CO 2 Culture in the incubator, and replace the culture medium every 2-3 days. The cells grew adherently in a single layer and were digested with 0.5% trypsin for passage. After the total number of cells reaches the requirement, trypan blue exclusion method determines that the number of viable cells is greater than 95%, and adjust the cell concentration to 5×10 6 / ml, cell suspension spare C57BL / 6 pure mice, male, 20 mice, 6-8 weeks old, body weight (18±1)g. Subcutaneous inoculation in the armpit. Each seed is inoculated with 0.2ml, cell count is 5×10 6 Cells / ml. The mice were randomly divided into 2 groups with 10 mice in each group. The control group was injected with 2 ml of normal saline, and the experimental group was injected with the T-2 toxin dissolved i...

Embodiment 3

[0021] Example 3: Experimental study on the therapeutic effect of T-2 toxin on liver cancer

[0022] Use mouse H 22 Hepatocellular carcinoma cell line, passaged in the abdominal cavity of ICR mice. Under aseptic conditions, extract the abdominal cavity and keep the seeds for 7-9 days. The H 22 For liver cancer cells, adjust the cell concentration to 5×10 with saline 6 / ml, 20 detergent ICR mice, 6-8 weeks old, male, 18-22g, randomly divided into 2 groups, each with 10 mice. 0.2ml of tumor cell suspension was inoculated subcutaneously in the left armpit. Seven days after the inoculation, the control group was injected with 2 ml of normal saline, and the experimental group was injected with the T-2 toxin dissolved in the ethanol-normal saline mixture at a dose of 2.0 mg / kg body weight. After group administration, the long diameter (L) and short diameter (W) of the tumor were measured with a vernier caliper every other day, and the tumor volume and tumor growth inhibition rate were...

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Abstract

The invention discloses new application of T-2 toxin, namely application of T-2 toxin to preparing drugs for treating pancreatic carcinomas. The effective treatment amount of T-2 toxin is 0.1-20mg / Kg, preferably 0.5-8mg / Kg. The drugs are administrated by implantation, oral administration, intravenous injection, hypodermic injection and intramuscular injection, preferably intra-tumor injection. The inventor builds various solid tumor models and then examines the inhibiting effect of T-2 toxin on the tumors or tumor cells by carrying out intra-tumor injection or culture with cells on T-2 toxin.Experiments show that intra-tumor injection has confirmed curative effect on the tumors, especially the solid tumors hard to excised and drug administration through intra-tumor injection can further reduce the side effect range and degree of T-2 toxin, reduce the side effects of T-2 toxin and improve the inhibiting and killing effects of T-2 toxin on the tumor tissues.

Description

Technical field [0001] The invention relates to the application of T-2 toxin in the preparation of medicines for treating pancreatic cancer. Background technique [0002] Tumors are one of the common diseases threatening human health, second only to cardiovascular diseases. The research on tumors has made great progress, and various methods of radiotherapy and chemotherapy have been developed to treat tumors. However, various drugs and therapies All have limitations, it is difficult to achieve a radical cure, and drug resistance and side effects are prone to occur. Tumor mortality still ranks first among various diseases. Statistics show that 3 million people died of cancer in my country in 2006, and the incidence of tumors is still on the rise, and there is a trend of getting younger. According to statistics, in less than 20 years, the incidence of cancer in my country has increased by 69%, and the mortality rate has increased by 29.4%. Therefore, research on new and effectiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00
Inventor 厉保秋
Owner SHENZHEN ICARBONX INTELLIGENT PEPTIDE PHARM TECH CO LTD
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