Application of three biflavone monomer components extracted from ginkgo leaves in preparing medicament of alpha-glucosidase inhibitor

A technology of ginkgo biflavonoids and glucosidase, which is applied in the field of medicine and can solve the problems of undiscovered ginkgo biflavonoids and the like

Inactive Publication Date: 2011-03-30
赵全成
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also do not find the report of the application of ginkgo biflavanone, different ginkgo biflavone and 7-demethyl ginkgo biflavone in the medicine of preparation α-glucosidase inhibitor

Method used

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  • Application of three biflavone monomer components extracted from ginkgo leaves in preparing medicament of alpha-glucosidase inhibitor
  • Application of three biflavone monomer components extracted from ginkgo leaves in preparing medicament of alpha-glucosidase inhibitor
  • Application of three biflavone monomer components extracted from ginkgo leaves in preparing medicament of alpha-glucosidase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 , Preparation of Ginkgo Biflavone, Iso-Ginkgo Biflavone and 7-Demethyl Ginkgo Biflavone

[0052] Take 1kg of dried ginkgo leaves, add 8000ml of 90°C water to soak for 3 times, each time for 3 hours, filter, and discard the filtrate. Add 8000ml of 95% ethanol to the soaked ginkgo leaves for 3 hours, then add 6000ml of 75% ethanol to extract twice, each time for 2 hours, filter, combine the filtrates, directly pass through the treated macroporous resin column, and use 75% Elute 3BV with ethanol, collect the effluent and eluate, recover ethanol, dry, add 250ml of petroleum ether for ultrasonic extraction 3 times, filter, discard the petroleum ether, dissolve the filter residue with 80% ethanol, recrystallize, and dry to obtain the total Biflavones. Get the total biflavonoids of Ginkgo biloba, dissolve them with methanol-0.1% acetic acid aqueous solution (70:30), separate and purify them on a C-18 reverse-phase column (atmospheric pressure or medium and low pres...

Embodiment 2

[0053] Example 2 , Preparation of Ginkgo Biflavone, Iso-Ginkgo Biflavone and 7-Demethyl Ginkgo Biflavone

[0054] Take 1kg of dried ginkgo leaves, add 8000ml of 90°C water to soak for 3 times, each time for 3 hours, filter, and discard the filtrate. Ginkgo biloba leaves soaked in water were dried, and 8 times the amount of 75% ethanol was added to reflux 3 times, each time for 3 hours, filtered, and the filtrate was combined, directly passed through the treated macroporous resin column, and 4BV was eluted with 75% ethanol, collected The effluent and the eluate were recovered with ethanol, dried, added 300ml of petroleum ether and ultrasonically extracted 3 times, filtered, discarded the petroleum ether liquid, dissolved the filter residue with 80% ethanol, recrystallized, and dried to obtain the total biflavonoids of Ginkgo biloba leaves. Get the total biflavonoids of Ginkgo biloba, dissolve them with methanol-0.1% acetic acid aqueous solution (70:30), separate and purify th...

Embodiment 3

[0055] Example 3 , Preparation of Ginkgo Biflavone, Iso-Ginkgo Biflavone and 7-Demethyl Ginkgo Biflavone

[0056] Take 1kg of dried ginkgo leaves, add 8000ml of 60°C water to soak for 3 times, each time for 3 hours, filter, and discard the filtrate. The ginkgo leaves soaked in water were dried, and 8 times the amount of 80% ethanol was added to reflux 3 times, each time for 3 hours, filtered, and the filtrate was combined, directly passed through the polyamide resin column that had been treated, and 3BV was eluted with 80% ethanol, and collected The effluent and the eluate were recovered with ethanol, dried, added 500ml of petroleum ether and ultrasonically extracted 3 times, filtered, discarded the petroleum ether liquid, dissolved the filter residue with 90% ethanol, recrystallized, and dried to obtain the total biflavonoids of Ginkgo biloba leaves. Get the total biflavonoids of Ginkgo biloba, dissolve them with methanol-0.1% acetic acid aqueous solution (70:30), separate ...

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Abstract

The invention provides three biflavone compounds, namely ginkgetin, isoginkgetin and 7-bilobetin from dry leaves of ginkgo biloba L. Pharmacological tests prove that the three biflavone compounds have the activity of inhibiting alpha-glucosidase. The invention discloses novel application of the ginkgetin, the isoginkgetin and the 7-bilobetin in preparing the alpha-glucosidase inhibitor, and also discloses a novel method for preparing the three biflavone compounds.

Description

technical field [0001] The invention relates to a method for extracting ginkgo biflavone, iso-ginkgo biflavone and 7-demethyl ginkgo biflavone from ginkgo leaves, all of which have new medical applications as proved by pharmacological experiments. Specifically, the application of ginkgo biflavone, iso-ginkgo biflavone and 7-demethyl ginkgo biflavone as alpha-glucosidase inhibitors in the preparation of hypoglycemic drugs. It belongs to the field of medical technology. Background technique [0002] Diabetes mellitus is a group of clinical syndromes caused by the interaction of genetic and environmental factors. It is a series of metabolic disorders in the body such as sugar, fat, protein, water and electrolytes caused by insufficient insulin secretion or impaired insulin action. The clinical symptom is hyperglycemia. Main common sign. It is manifested as an abnormal increase in the concentration of glucose in the blood and urine. When the blood sugar and urine sugar are too...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P3/10
Inventor 赫玉芳赵全成陈声武南敏伦马吉胜赵文杰张瑜王婵
Owner 赵全成
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