Vibrio alginolyticus strain and application thereof
A technology of Vibrio alginolyticus and strains, applied in the field of probiotics, can solve problems such as unsuitable for proliferation, achieve the effects of improving non-specific immunity, enhancing infectivity, and improving the ability of prawns to resist viral pathogen infection
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[0020] Example 1:
[0021] The preparation method of Vibrio alginolyticus SSL073 powder is as follows:
[0022] Pick a small amount of strains stored at -80°C and streak it on 2216E solid medium, culture at 28°C for 24 hours to obtain a single colony, then inoculate it in the seed liquid, shake flask culture, and then inoculate the seed liquid in the fermentation broth for fermentation culture. The fermentation temperature is controlled at 25-28°C; when the OD600 value of the fermentation broth reaches 1.6, the fermentation is terminated; the fermentation broth is obtained; the fermentation broth is centrifuged and concentrated 20-50 times to obtain a turbid broth. Add the lysis solution to 1 / 5-1 / 10 of the volume of the turbid liquid to resuspend the bacteria, digest at 37°C for 24 hours; 300w, 10s / 10s ultrasonic wave breaks for 20 minutes, and the broken bacterial liquid is spray dried (air inlet and air outlet The temperatures were at 195°C and 85°C, respectively, to prepare Vib...
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[0023] Example 2:
[0024] Application of Vibrio alginolyticus SSL073 powder:
[0025] 10 grams of Vibrio alginolyticus powder and 85 grams of auxiliary protective carrier corn starch are uniformly mixed to prepare a composite probiotic preparation.
[0026] Add the compound probiotic preparation to 10 kg of basic shrimp feed ingredients (containing 30% fish meal, 10% shrimp meal, 25% soybean meal, 25% peanut meal, 3% flour, 3% corn meal, and 1% compound vitamins) , Formulated into immune feed containing anti-disease microorganism preparations. The various raw materials were crushed through a 60-mesh sieve, accurately weighed, and mixed step by step. Sodium alginate was used as a binder, and a small amount of water was added to squeeze into a pellet feed. The feed was baked at 40°C. Dried until the moisture content is below 10%, packaged in bags, and stored in a refrigerator at 4°C for later use.
[0027] More than 800 healthy Litopenaeus vannamei, body length (6.80±0.38) cm, were r...
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