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Method for extracting porphyra polysaccharide and porphyra protein

A technology of laver polysaccharides and extraction methods, applied in food preparation, food science, applications, etc., can solve the problems of low extraction yield, weak competitiveness, and low added value of laver products, achieve high biological activity, increase economic value and The effect of social value

Inactive Publication Date: 2011-04-06
刘奕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the utilization of laver resources in my country basically belongs to the direct utilization of raw materials, and there is no industrialized processing of laver protein. It also contains a large amount of polysaccharides, the two are combined with each other, and it is not easy to be separated
Therefore, the added value of laver products is low, the competitiveness is weak, and the economic benefits are poor, which greatly affects the comprehensive development of laver resources.

Method used

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  • Method for extracting porphyra polysaccharide and porphyra protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Drying and pulverization: Bake the seaweed at 50°C for 12 hours, and pulverize it to make seaweed dry powder.

[0024] (2) Alkali-soluble, ultrasonic technology-assisted extraction: pH is 8, the solution dosage is 40 times that of the dried seaweed powder, the ultrasonic temperature is 50°C, the time is 30min, and the power is 150W.

[0025] (3) High-speed centrifugation: the extraction liquid was centrifuged at high speed for 10 min under the condition of 4000 r / min, the supernatant was used to prepare laver protein, and the precipitation was used to extract laver polysaccharide.

[0026] (4) Isoelectric point precipitation: adjust the pH of the centrifuged supernatant to 5, and after standing for 15 minutes, centrifuge at a high speed for 10 minutes under the condition of 4000 r / min, the resulting precipitate is porphyrin, and the supernatant is a polysaccharide extract .

[0027] (5) Ultrafiltration and purification of protein: The protein obtained by isoelectri...

Embodiment 2

[0033] (1) drying, pulverizing: same as Example 1.

[0034] (2) Alkali-soluble, ultrasonic technology-assisted extraction: pH is 8.5, the solution dosage is 60 times that of the dried seaweed powder, the ultrasonic temperature is 30°C, the time is 45min, and the power is 200W.

[0035] (3) high-speed centrifugation: same as Example 1.

[0036] (4) Isoelectric point precipitation: adjust the pH of the centrifuged supernatant to 4, and after standing for 22 min, centrifuge at a high speed for 10 min under the condition of 4000 r / min, the obtained precipitate is porphyrin, and the supernatant is a polysaccharide extract .

[0037] (5) Ultrafiltration and purification of protein: The protein obtained by isoelectric precipitation was prepared into a 5% solution with purified water, and a membrane with a molecular weight cut-off of 10,000 Da was used for separation and purification. The feed pressure is 0.15MPa, the pH is 8, the temperature is 35°C, and the tangential flow rate is...

Embodiment 3

[0043] (1) drying, pulverizing: same as Example 1.

[0044] (2) Alkali-soluble, ultrasonic technology-assisted extraction: pH is 9, the solution dosage is 20 times that of the dried seaweed powder, the ultrasonic temperature is 40°C, the time is 60min, and the power is 100W.

[0045] (3) high-speed centrifugation: same as Example 1.

[0046] (4) Isoelectric point precipitation: adjust the pH of the centrifuged supernatant to 4.5, and after standing for 30 min, centrifuge at a high speed for 10 min under the condition of 4000 r / min, the resulting precipitate is porphyrin, and the supernatant is a polysaccharide extract .

[0047] (5) Ultrafiltration and purification of protein: The protein obtained by isoelectric precipitation was prepared into a 3% solution with purified water, and a membrane with a molecular weight cut-off of 20,000 Da was used for separation and purification. The feed pressure is 0.2MPa, the pH is 7, the temperature is 45°C, and the tangential flow rate is...

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Abstract

The invention discloses a method for extracting porphyra polysaccharide and porphyra protein, belonging to the technical field for extracting natural active constituents. The method comprises the following steps of: after drying and grinding porphyra, extracting protein and polysaccharide with dilute alkali liquor auxiliary with ultrasonic waves; precipitating the protein in an isoelectric precipitation method, purifying by ultrafiltration, and obtaining a porphyra protein product by spray drying; cleaning residuals after the protein is extracted into neutrality; extracting polysaccharide by dynamic ultrahigh pressure, decolorizing with active carbon, purifying by ultrafiltration, and obtaining a polysaccharide product after spray drying. The method adopts the ultrasonic waves and a dynamic ultrahigh pressure technique to increase the extraction ratio of protein and polysaccharide, and adopts an active carbon decolorizing and ultrafiltration technique to increase the purity of protein and polysaccharide. The residuals after extraction can be cleaned and purified to be prepared into insoluble dietary fiber products, and therefore the porphyra raw material is completely used. The extracted porphyra protein and porphyra polysaccharide have higher bioactivity and can be widely applied in industries, such as biomedicine, cosmetics, food and the like. The economic value and the social benefits of porphyra are improved.

Description

technical field [0001] The invention relates to an extraction method of laver polysaccharide and laver protein, belonging to the technical field of natural active ingredient extraction. Background technique [0002] my country is a big country in seaweed resources, production, processing and utilization. my country's seaweed output ranks first in the world, and coastal provinces such as Zhejiang, Fujian, Guangdong, and Jiangsu are all planted. According to incomplete statistics, the annual output of seaweed in the country is about 60,000 tons, with a total value of nearly 10 billion yuan, and there are 300,000 related employees. The products are exported to Japan, Southeast Asia, Europe and the United States and other countries and regions. However, at present, domestic seaweed products are mainly in the rough processing stage, that is, processed into rough processed food or seasonings with relatively low added value through simple drying methods, and the price is only 26-40...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23J1/00A23L1/305A23L1/09A23L29/30A23L33/185
Inventor 刘奕
Owner 刘奕
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