Method for extracting porphyra polysaccharide and porphyra protein

Abstract

The invention discloses a method for extracting porphyra polysaccharide and porphyra protein, belonging to the technical field for extracting natural active constituents. The method comprises the following steps of: after drying and grinding porphyra, extracting protein and polysaccharide with dilute alkali liquor auxiliary with ultrasonic waves; precipitating the protein in an isoelectric precipitation method, purifying by ultrafiltration, and obtaining a porphyra protein product by spray drying; cleaning residuals after the protein is extracted into neutrality; extracting polysaccharide by dynamic ultrahigh pressure, decolorizing with active carbon, purifying by ultrafiltration, and obtaining a polysaccharide product after spray drying. The method adopts the ultrasonic waves and a dynamic ultrahigh pressure technique to increase the extraction ratio of protein and polysaccharide, and adopts an active carbon decolorizing and ultrafiltration technique to increase the purity of protein and polysaccharide. The residuals after extraction can be cleaned and purified to be prepared into insoluble dietary fiber products, and therefore the porphyra raw material is completely used. The extracted porphyra protein and porphyra polysaccharide have higher bioactivity and can be widely applied in industries, such as biomedicine, cosmetics, food and the like. The economic value and the social benefits of porphyra are improved.

Description

technical field

[0001] The invention relates to an extraction method of laver polysaccharide and laver protein, belonging to the technical field of natural active ingredient extraction. Background technique

[0002] my country is a big country in seaweed resources, production, processing and utilization. my country's seaweed output ranks first in the world, and coastal provinces such as Zhejiang, Fujian, Guangdong, and Jiangsu are all planted. According to incomplete statistics, the annual output of seaweed in the country is about 60,000 tons, with a total value of nearly 10 billion yuan, and there are 300,000 related employees. The products are exported to Japan, Southeast Asia, Europe and the United States and other countries and regions. However, at present, domestic seaweed products are mainly in the rough processing stage, that is, processed into rough processed food or seasonings with relatively low added value through simple drying methods, and the price is only 26-40...

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