Method for extracting and detecting nitrate and nitrite in Chinese sauerkraut

A technology of nitrite and nitrate, applied in the field of extraction and detection, can solve the problems of long time, long time, low efficiency and the like

Inactive Publication Date: 2011-04-13
HARBIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of long time, low efficiency and complex operation in the existing method for extracting nitrate and nitrite in sauerkraut, and the detection method has the disadvantages of complicated process, long time consumption and narrow linear range.

Method used

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Experimental program
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specific Embodiment approach 1

[0011] Specific embodiment one: the method for extracting nitrate and nitrite in sauerkraut in the present embodiment is carried out according to the following steps: sauerkraut is minced, then gets 1g and joins 2.5mL borax saturated solution through the sauerkraut after the minced processing Stir evenly, add 10mL of distilled water, then extract by microwave heating for 5min, the temperature of microwave heating is 80°C, then add 1mL of potassium ferrocyanide solution with a concentration of 0.25mol / L and 1mL of zinc acetate solution with a concentration of 1mol / L, Constant volume, static filtration, collecting the filtrate to obtain the extract; that is to complete the extraction of nitrate and nitrite in sauerkraut.

[0012] In this embodiment, the extraction rates of nitrate and nitrite are both above 90%. The measured content of nitrite in the extracted sauerkraut is 3.95mg / kg, which is 41.6% higher than the national standard method (2.79mg / kg).

specific Embodiment approach 2

[0013] Embodiment 2: This embodiment differs from Embodiment 1 in that: the time for grinding sauerkraut is 2 minutes, and the rotating speed used is 1400 r / min.

specific Embodiment approach 3

[0014] Specific embodiment three: detect nitrate and nitrite in sauerkraut in the present embodiment and complete according to the following steps: one, the preparation of luminol analysis solution: draw 7.5mL concentration and be 1 * 10 -3 mol / L luminol solution, be that the NaOH solution constant volume 50mL of 0.25mol / L obtains luminol analysis liquid with concentration; Two, the mensuration of nitrite: get 5.00mL from pickled cabbage Extracted extract, then add 10.00mL potassium bromate solution with a concentration of 0.06mol / L, 3.00mL with a concentration of 5×10 -3 mol / L ethylenediaminetetraacetic acid (EDTA) solution and 2.50mL concentration of 0.5mol / L H 2 SO 4 Solution, constant volume 50mL, to obtain the sample solution A to be tested, add 2.00mL of the luminol analysis solution prepared in step 1 and 2.00mL of the sample solution A to be tested in sequence in the transient static chemiluminescence instrument for measurement, and record the luminescence peak of the...

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Abstract

The invention discloses a method for extracting and detecting nitrate and nitrite in Chinese sauerkraut and relates to an extracting and detecting method. The invention solves the problems of long time, low efficiency and complex operation existing in the traditional method for extracting nitrate and nitrite in Chinese sauerkraut and overcomes the defects of complex process, long time, narrow linear range, and the like existing in the method for nitrate and nitrite in Chinese sauerkraut. A microwave method is used for assisting to extract nitrate and nitrite in the Chinese sauerkraut. The nitrate is reduced by utilizing a zinc-cadmium water bath oscillating method, and the nitrate and the nitrite are detected by adopting a photochemical method. The extracting method of the invention has the characteristics of simple process, short time, high extracting efficiency, simple equipment, wide application range, high repeatability, time saving, reagent saving, less pollution, and the like. The detecting method of the invention has the advantages of simple process, short time, high sensitivity, wide linear range and simple instrument equipment.

Description

technical field [0001] The invention relates to a method for extraction and detection. Background technique [0002] Sauerkraut, as a common pickled vegetable in the north, uses cabbage as raw material and is stored by pickling to supplement the lack of winter vegetables. During the pickling process, the fermentation is carried out with the help of certain microorganisms naturally attached to the surface of the vegetables, and the nitrate in the vegetables will be reduced to nitrite under the action of reducing bacteria. Research has shown that [1] , During the pickling process of vegetables, the content of nitrite showed a trend of rising first and then falling. When people eat sauerkraut with excessive nitrite content, they will suffer from nitrite poisoning. In mild cases, dizziness, heart palpitations, vomiting, and blue lips. In addition, nitrite reacts with secondary amines in the human body to form nitrosamines [2] , it is a carcinogenic, teratogenic and mutagenic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B21/48C01B21/50G01N21/76
Inventor 李健黎晨晨刘宁陈姝娟
Owner HARBIN UNIV OF COMMERCE
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