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Chitosan nano particle and preparation method thereof

A technology of chitosan nano and chitosan, which is applied in the direction of pharmaceutical formulations, medical preparations of non-active ingredients, powder delivery, etc., can solve the problems of large particle size of microspheres, restricted administration routes, and complicated preparation process, etc. Achieve the effects of simple preparation method, meet the needs of production and application, and simple preparation process

Inactive Publication Date: 2011-04-13
SHANGHAI NAT ENG RES CENT FORNANOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Often these preparations are complex and require organic solvents or surfactants
In addition, the size of microspheres prepared by these techniques is relatively large, which limits certain routes of administration, such as intravenous injection

Method used

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  • Chitosan nano particle and preparation method thereof
  • Chitosan nano particle and preparation method thereof
  • Chitosan nano particle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of chitosan nanoparticles using different ratios of chitosan / polyacrylic acid

[0034] 1. Preparation of chitosan solution / polyacrylic acid solution:

[0035] a. Chitosan is formulated into acetic acid solutions with concentrations of 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg / ml;

[0036] b. Polyacrylic acid (PAA) was formulated into aqueous solutions with concentrations of 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, and 0.06%, respectively.

[0037] c. Preparation of chitosan nanoparticles: add the above polyacrylic acid solution dropwise into the chitosan solution according to the volume ratio of 1:5. Specifically, 1 ml of PAA (molecular weight: 100 kDa) aqueous solution was added dropwise into 5 ml chitosan solution (chitosan with molecular weight of 80 kDa was dissolved in 1% (w / v) acetic acid solution), and magnetic Stir to form a milky white suspension. The resulting milky white suspension was filtered with filter paper, and the filtered suspension wa...

Embodiment 2

[0039] Example 2 Incubation of Chitosan Nanoparticles Using Buffers of Different pH

[0040] a. According to the volume ratio of 1:5, 1 ml of 0.02% PAA (molecular weight is 100 kDa) aqueous solution is added dropwise in the chitosan solution of 5 ml 0.2 mg / ml (the chitosan with molecular weight of 80 kDa is dissolved in 1% (w / v) acetic acid solution) while magnetically stirring to form a milky white suspension. The resulting milky white suspension was filtered with filter paper, and the filtered suspension was allowed to stand for 24 hours.

[0041] b. Prepare buffer solutions with different pH values ​​to make them pH=1, 2, 3, 4, 5, 6, 7,9.

[0042] c. Incubate the chitosan nanoparticles prepared above in buffer solutions with pH=1, 2, 3, 4, 5, 6, 7, and 9 for 24 hours.

[0043] Such as Figure 5 The results showed that the chitosan nanoparticles were stable in the pH range of 3.0-7.0, and the particle size of the chitosan nanoparticles increased with the increase of the...

Embodiment 3

[0044] Embodiment 3 places the chitosan nanoparticle of different time

[0045] a. Prepare chitosan into an acetic acid solution with a concentration of 0.2 mg / ml, and polyacrylic acid (PAA) into an aqueous solution with a concentration of 0.02%.

[0046] b. Add 1 ml of 0.02% PAA (molecular weight: 100 kDa) aqueous solution dropwise into 5 ml of 0.2 mg / ml chitosan solution (chitosan with a molecular weight of 80 kDa is dissolved in 1% (w / v) acetic acid solution).

[0047] c. Simultaneously stir magnetically to form a milky white suspension.

[0048] d. Leave them for 1 day, 10 days, 20 days, 30 days, 60 days.

[0049] Such as Figure 6 , 7 The results show that within 60 days, the particle size and surface potential change trends of chitosan nanoparticles are almost identical, which can explain to a certain extent that the chitosan nanoparticle system prepared by this method is relatively stable and suitable for biological systems. among.

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Abstract

The invention relates to a chitosan nano particle and a preparation method thereof. The nano particle is a chitosan nano particle synthesized from chitosan and polyacrylic acid, and is characterized in that the nano particle is a spherical particle of which the particle diameter is 150-200 nm with a tolerance of + / - 20 nm, and the surface potential is 40-60 mv. The prepared chitosan nano particle can be well applicable to multiple fields, such as age-related macular degeneration, pigmentary degeneration of retina, diabetic retinopathy, glaucoma, cataract, other ocular diseases and the like. Besides, the invention has the advantages of simple preparation process and low production cost, and the prepared chitosan nano particle has high stability, thereby further meeting the production and application requirements.

Description

technical field [0001] The invention relates to a chitosan nano particle and a preparation method thereof. Background technique [0002] Chitosan (Chitosan) is an important class of natural biodegradable polymer materials. Existing in the cell wall of fungal yeast, it is the only cationic edible animal fiber containing free amino basic groups in nature. Its molecular structure is similar to cellulose, and it is a straight-chain polymer formed by glucosamine linked by glycosidic bonds, including glucosamine copolymer and N-acetylglucosamine. Chitosan has been proven to have many excellent properties: non-toxicity, permeability enhancement, bioadhesion, good tissue compatibility, biodegradability, easy to be prepared into microparticles and nanoparticles. In addition, because it also has health care products such as anti-cancer and blood fat reduction. Since the 1980s, there has been an upsurge in the development and research of polysaccharide biomedical materials such as c...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08L33/02C08J3/12A61K9/14A61K47/36
Inventor 王美艳黄潇金彩虹何丹农魏锐利
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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