Processes of producing alcohols
An ethanol, bioreactor technology, applied in the direction of microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve problems such as lower efficiency than required
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Embodiment 1
[0169] Example 1: Effect of pH and Oxidation-Reduction Potential (ORP) Levels on the Production of Ethanol Using CO-Containing Gas:
[0170] The experiments described in this example show the kinetics of ethanol production by Clostridium autoethanogenum cultures in fermentors at different pH and ORP levels with continuous input of CO as the sole carbon and energy source.
[0171] step
[0172] 1. An actively growing Clostridium autoethanogenum culture (DSMZ 10061 ) was inoculated into 2L of anaerobic LM23 fermentation medium at a level of 7.5% (v / v). 95% CO and 5% CO 2 A continuous stream of air was introduced into the bottom of the fermenter vessel through a diffuser injector at a volumetric flow rate of 5 ml / min. The initial pH of the fermentor was set at 5.5.
[0173] 2. The culture was grown to a bacterial cell density of 0.1 g dry cell mass per liter of medium at which point the pH and ORP of the culture were adjusted and ethanol production was monitored.
[0174] 3. ...
Embodiment 2
[0182] Example 2: Monitoring the intracellular redox potential of microbial cultures
Embodiment 2A
[0183] Example 2A: Procedure for Monitoring Intracellular Redox Potential and Cell Viability of Batch Microbial Cultures
[0184] 1. Prepare LM23 fermentation medium at pH 5.5 as described above.
[0185] 2. At 95% CO and 5% CO 2 Under continuous airflow, inject 50ml LM23 medium into the 250ml serum bottle. All bottles were stoppered with airtight butyl rubber septa and crimp sealed before being sterilized at 121°C for 20 minutes.
[0186] 3. Once cooled, divide the serum bottles into two groups. In one group, methyl viologen was added to the medium to a final concentration of 40 μg / ml. Check the pH of all bottles and adjust to 5.5 if necessary. All bottles were inoculated with 1ml at 95% CO and 5% CO 2 Actively growing Clostridium autoethanogenum cultures in . 95% CO and 5% CO for headspace gas 2 Pressurize to 35 psig. Aseptically withdraw a sample of starting medium from each bottle. Place the bottle on a shaker at 37°C.
[0187] 4. Media samples were taken at regu...
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