Protein, coding genes and application thereof

Abstract

The invention discloses a protein, coding genes and an application thereof. The protein is artificially synthesized and is named as KcPCS1, and is the protein as the following (1) or (2): (1) the protein consisting of amino acid sequences expressed by a sequence 2 in a sequence list; (2) the protein which is derived from (1) by substituting and / or deleting and / or adding the amino acid sequences in the sequence 2 by one or more amino acid residues and is related to stress tolerance. The experiments show that KcPCS1 gene is subjected to yeast transformation; the transgenic yeast has excellent resistance to cadmium (Cd); and the content of Cd in the body is higher than that of the yeast cell transferring into a vector.

Description

technical field

[0001] The invention relates to a protein, its coding gene and application. Background technique

[0002] Heavy metals generally refer to specific gravity greater than 4 or 5, density greater than 4.5g / cm 3 There are about 45 kinds of metals, such as copper, lead, zinc, iron, cobalt, nickel, vanadium, niobium, tantalum, titanium, manganese, cadmium, mercury, tungsten, molybdenum, gold, silver and other metals. Although arsenic is a non-metal element, it is often classified as a heavy metal because of its high toxicity.

[0003] Excessive heavy metals have a strong toxic effect on plants and can affect the growth and development of plants. Chen Qinghua et al. used heavy metal Cr 6+ Maize seedlings were treated, and it was found that the malondialdehyde (MDA) and electrical conductivity in the plant were significantly correlated with the concentration of Cd, while the chlorophyll content and superoxide dismutase (SOD) increased with the increase of treatment...

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