Method for identifying radix rosae gigantea extract
A technology for the root and extract of G. kung fu, which is applied in the field of identification of G. kung fu root extracts, can solve problems such as poor specificity, differences in quality, and difficulty
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Embodiment 1
[0029] Embodiment 1: A kind of identification method of the root extract of Gongguo
[0030]1. Instruments and reagents
[0031] 1.1 Instrument
[0032] Shimadzu high performance liquid chromatograph (LC-20AT double pump, SPD-M20AUV detector, CTO-20A column thermostat, Japan Shimadzu Corporation), SHIMADZU VP-ODS column (250×4.60mm, 5micron).
[0033] 1.2 Reagent
[0034] The catechin reference substance was provided by SIGMA Company; the methanol, ethanol, hydrochloric acid, glacial acetic acid, etc. used for extraction were all analytically pure; the methanol and acetonitrile used for liquid phase analysis were all chromatographically pure. Gugu root is collected from Yunnan Province, self-made extract, batch number: 20050918, 20051009, 20061123, 20061128, 20071021, 20071029.
[0035] 2. Methods and results
[0036] Selection of chromatographic column: Under the same chromatographic conditions, we investigated: Ultimate XB-C18 (150mm×4.6mm, 5μm) column, SHIMADZU VP-ODS c...
Embodiment 2
Embodiment 3
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