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Research and application of quartz fluorescent sensor for field rapid in-vitro instant multi-disease synchronous diagnosis based on immune nanocrystalline

A fluorescent sensor, nanocrystal technology, applied in the fields of resistance to vector-borne diseases, fluorescence/phosphorescence, instruments, etc., can solve the problem of inability to meet the requirements of rapid diagnosis and monitoring of the epidemic site, and achieve simple diagnostic monitoring steps, improve sensitivity, and improve Effects of Accuracy and Sensitivity

Inactive Publication Date: 2013-08-14
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the previous immunodiagnostic technology was still carried out in the laboratory, relying on the heavy equipment of the laboratory, which could not meet the requirements of rapid diagnosis and monitoring on the scene of the epidemic

Method used

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  • Research and application of quartz fluorescent sensor for field rapid in-vitro instant multi-disease synchronous diagnosis based on immune nanocrystalline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 (Class A infectious disease, such as plague, cholera)

[0047] A method for preparing an immune nanocrystalline quartz fluorescent sensor for on-site rapid in vitro instant diagnosis and monitoring of plague and cholera, comprising the following steps:

[0048] (1) Extract plague pathogen-related proteins or specific markers as antigens from body fluids, blood or secretion samples; extract cholera pathogen-related proteins or specific markers as antigens from body fluids, blood or secretion samples; Antibodies corresponding to the above antigens were prepared by in vivo injection.

[0049] (2) Prepare two kinds of CdSe / CdS nanocrystal solutions with different particle sizes: 2 Under protection, the Se powder was dissolved in trioctylphosphine to prepare the Se precursor, and the S powder was dissolved in trioctylphosphine to prepare the S precursor, and then the prepared Cd(OA) 2 , reaction, under the condition of oleic acid as a stabilizer, two kinds of ...

Embodiment 2

[0054] Embodiment 2 (Class B infectious diseases, such as infectious atypical pneumonia, AIDS, viral hepatitis)

[0055] A method for preparing an immune nanocrystalline quartz fluorescent sensor for on-site rapid in vitro instant simultaneous diagnosis and monitoring of infectious atypical pneumonia, AIDS, and viral hepatitis, comprising the following steps:

[0056] (1) Extracting SARS-related pathogen-related proteins or specific markers as antigens from body fluid, blood or secretion samples. AIDS pathogen-related proteins or specific markers are extracted from body fluid, blood or secretion samples as antigens. Extract viral hepatitis pathogen-related proteins or specific markers as antigens in body fluid, blood or secretion samples. Antibodies corresponding to the above antigens are then prepared by in vivo injection in animals.

[0057] (2) Prepare three kinds of CdTe / CdS nanocrystal solutions with different particle sizes: 2 Under protection, Te powder was dissolved...

Embodiment 3

[0062] Embodiment 3 (Class B infectious diseases, such as poliomyelitis, human infection highly pathogenic avian influenza, measles, epidemic hemorrhagic fever)

[0063] A method for preparing an immune nanocrystalline quartz fluorescent sensor for on-site rapid in vitro instant diagnosis and monitoring of polio, human infection with highly pathogenic avian influenza, measles, and epidemic hemorrhagic fever, comprising the following steps:

[0064] (1) Poliomyelitis pathogen-related proteins or specific markers are extracted from body fluid, blood or secretion sample solutions as antigens. Extract human infection highly pathogenic avian influenza pathogen-related proteins or specific markers as antigens in body fluids, blood or secretions. Measles pathogen-related proteins or specific markers are extracted from body fluids, blood or secretions as antigens. Extracting epidemic hemorrhagic fever pathogen-related proteins or specific markers as antigens from body fluids, aspergi...

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Abstract

The invention discloses an immune nanocrystalline quartz fluorescent sensor for field rapid in-vitro instant multi-disease synchronous diagnosis and a method for diagnosing and monitoring infectious diseases. A preparation method of a immune nanocrystalline comb multi-probe quartz plate, as shown in the figure, comprises the following steps: selecting the pathogen related protein of the infectious diseases or a marker thereof as antigen; preparing the nanocrystalline and preparing immune nanocrystalline according to the document; and modifying the immune nanocrystalline of different antibodies on the surfaces of different probes of the comb quartz plate by using the layer-by-layer surface modification technique. A multi-disease synchronous diagnosis monitoring method comprises the following steps: soaking the modified quartz plate in an environment sample solution, body fluid, blood or secretion sample; placing in a sealed quartz excitation vessel transmitting light path; and performing diagnosis monitoring on the infectious diseases. The invention has the advantages of high specificity, high sensitivity, short diagnosis monitoring time and low cost.

Description

technical field [0001] The present invention relates to the technical field of on-site rapid in vitro real-time simultaneous diagnosis and monitoring of multiple diseases, and more specifically to the preparation of an immune nanocrystalline quartz fluorescent sensor for on-site rapid in vitro real-time simultaneous diagnosis and monitoring of multiple diseases. Immuno-nanocrystalline quartz fluorescent sensor for on-site rapid in vitro instant diagnosis and monitoring of Class A, B, and C infectious diseases. Background technique [0002] Infectious diseases are a class of diseases caused by various pathogens that can be transmitted between humans, animals and animals, or between humans and animals. Most of the pathogens are microorganisms, and a small part is parasites, which are also called parasitic diseases. Infectious diseases are characterized by the presence of pathogens, infectivity and prevalence, and often immunity after infection. Some infectious diseases are a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533G01N21/64
CPCY02A50/30
Inventor 于京华葛磊王寿梅颜梅杨萍黄加栋王珊张丛丛
Owner UNIV OF JINAN