Artificially cultured Grifulaumbelleta strain, Amillariella mellea and sclerotium production method for Grifulaumbelleta strain
A production method and technology of Armillaria are applied in the directions of biochemical equipment and methods, methods of using spores, microorganisms, etc., which can solve the problems of restricting Polyporus chinensis, rising cost of artificial cultivation, difficulty in artificial cultivation of provenance, etc., and achieve sclerotia. The effect of forming fast, satisfying large-scale production and low production cost
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Embodiment 1
[0023] 1. Prepare a primary medium with 10g corn flour, 20g sucrose, 3g beef extract, 22g agar, and 1000ml water; sterilize for 20 minutes at 1.5 atmospheres and 121°C;
[0024] 1) Inoculate the strain of Polyporus spp. in a sterile environment, the volume ratio of the strain of Polyporus spp. to the culture medium is 25:100, cultivate it at 20°C for 20 days to make a first-class strain, and form a large number of sclerotia on the inclined surface of the test tube;
[0025] 2) After sterilizing with the formula of the primary medium, cool the upright test tube at 25°C, transfer Armillaria armillaria, the volume ratio of the strain to the medium is 25:100, and the culture conditions are the same as that of Polyporus sp., to obtain Armillaria armillaria kind;
[0026] 2. Prepare a secondary culture medium with a water content of 60% by weight ratio of 20% corn squid and 80% eucalyptus wood chips. The corn squid is pre-soaked in water for 12-16 hours before use; then put the seco...
Embodiment 2
[0029] 1. Prepare a primary medium with 50g corn flour, 10g glucose, 1g beef extract, 15g agar, and 1000ml water; sterilize for 20 minutes at 1.5 atmospheres and 121°C;
[0030] 1) Inoculate the strain of Polyporus spp. in a sterile environment, the volume ratio of the strain of Polyporus spp. to the culture medium is 20:100, cultivate it at 27°C for 20 days to make a first-grade strain, and form a large number of sclerotia on the inclined surface of the test tube;
[0031] 2) After sterilizing with the formula of the primary medium, cool the upright test tube at 25°C, transfer to Armillaria armillaria, the volume ratio of the strain to the medium is 20:100, and the culture conditions are the same as that of Polyporus sp.;
[0032]2. Prepare the secondary culture medium with 55% water content with 60% corn squid, 40% broad-leaved maple and elm with a diameter of 0.1-2cm and 3-5cm long small wood section. Soak in water for 1 hour to pre-wet; then put the secondary culture mediu...
Embodiment 3
[0035] 1. Prepare a primary medium with 30g corn flour, 15g sucrose or glucose, 2g beef extract, 18g agar, and 1000ml water; sterilize for 20 minutes at 1.5 atmospheres and 121°C;
[0036] 1) Inoculate the strain of Polyporus spp. in a sterile environment, the volume ratio of the strain of Polyporus spp. to the culture medium is 16:100, cultivate it at 25°C for 18 days to make a first-class strain, and form a large number of sclerotia on the inclined surface of the test tube;
[0037] 2) After sterilizing with the first-grade medium formula, cool the upright test tube at 25°C, transfer to Armillaria armillaria, the volume ratio of strain to medium is 16:100, and the culture conditions are the same as that of Polyporus sp.;
[0038] 2. Prepare a secondary medium with a water content of 58% by weight ratio of 30% corn squash and 70% chestnut sawdust. Corn squash needs to be soaked in water and pre-wetted 12-16 hours in advance; then put the secondary medium into canned bottles A...
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