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A kind of nanoparticle composition and its application

A nanoparticle and composition technology, applied in the field of nanoparticle composition, can solve the problems of biological safety, limited clinical application of virus vectors, low gene transfer efficiency, etc., to prevent or treat malignant melanoma, not easy to degrade, and stable effect Effect

Inactive Publication Date: 2015-08-12
姚宏
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  • Claims
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Problems solved by technology

However, unintended host immune responses and potential biosafety issues elicited by viral vector surface proteins have limited the clinical application of viral vectors.
However, the gene delivery efficiency of currently used non-viral vectors is still lower than that of adenoviral vectors

Method used

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  • A kind of nanoparticle composition and its application
  • A kind of nanoparticle composition and its application
  • A kind of nanoparticle composition and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0040] 1. Establishment of experimental model: establish an animal model of melanoma.

[0041] Mouse melanoma B16-F1 cells (2×10 5 cells / mouse, one-time injection) were planted subcutaneously in immunocompetent mice C57BL / 6. After 7 days, the injection site can be seen to have a diameter of about 5×5mm 2 The bulge is confirmed as a tumor.

[0042] 2. Test the preventive or therapeutic effect of H1 / phIL2 nanocomposition on melanoma

[0043] (1) Pre-test: used to study the time, location and duration of gene expression to determine the best treatment strategy.

[0044] After the establishment of the melanoma cell animal model, the tumor-forming mice were randomly divided into 3 groups (N=3): a single injection of 50ug of the plasmid expressing the luciferase gene (pLuc); injection of H1 and a plasmid expressing the luciferase gene Low dose group (DNA25ug); injection of H1 and plasmid expressing luciferase gene group (DNA50ug). After injecting the above drugs around the tumo...

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Abstract

The invention provides a nano particle (H1 / phIL2) self-assembled by a polycation (H1) carrier and a plasmid for expressing human interleukin-2, and application of the nano particle in preparing medicines for treating, controlling or preventing cancers. The polycation (H1) carrier is a cyclodextrins-polyethyleneimine-folic acid ternary assembly polycation carrier.

Description

technical field [0001] The invention relates to a nanoparticle composition for preventing or treating cancer, in particular to a plasmid expressing human interleukin-2 cytokine or expressing human interferon alpha cytokine and a nanoparticle of a nanopolycation carrier (H1) Compositions and their applications. Background technique [0002] With the development of biomedical technology, biotherapeutic programs using biological agents to treat tumors are emerging. Compared with chemotherapy, biological therapy is more physiological, tolerable and effective for patients with advanced tumors. Currently, the most widely used tumor biotherapeutic agents are recombinant cytokines. Cytokines are a group of low-molecular-weight regulatory proteins with pleiotropic, receptor-specific and transient functional characteristics, and they are the signal regulation network of the immune system. Clinical and experimental data have confirmed that a variety of cytokines can stimulate the bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K9/14A61K47/40A61K47/34A61K47/22A61P35/00A61P35/04A61P17/00C12N15/21C12N15/26
Inventor 姚宏林李家宓
Owner 姚宏
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