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Process for extracting polysaccharide from umbellate pore fungus

An extraction process and a technology of total polysaccharides, which are applied in the direction of drug combinations, medical raw materials derived from fungi, allergic diseases, etc., can solve the problems of low extraction rate and long time consumption, and achieve high extraction rate, short time consumption and simple operation Effect

Inactive Publication Date: 2011-05-11
孔令敏
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  • Claims
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Problems solved by technology

[0003] The extraction method of polyporus polysaccharide is mainly water decoction extraction method, which not only takes a long time, but also has a low extraction rate

Method used

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  • Process for extracting polysaccharide from umbellate pore fungus
  • Process for extracting polysaccharide from umbellate pore fungus
  • Process for extracting polysaccharide from umbellate pore fungus

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Embodiment Construction

[0009] The process steps are: crush the Polyporus umbellatus crude drug, sieve 10-30 meshes with a sample sieve, add distilled water with a material-to-water ratio of 1:7 to 1:9 to the Polyporus umbellatus powder, soak for 24 hours; extract with a Soxhlet extractor 7-9h; the filtrate is obtained by suction filtration, the material-to-liquid ratio of crude drug and water body fluid in the filtrate is 1:1~1:3, 65-95% ethanol is added, and the material-to-liquid ratio is 1:4~1:6. Let it stand overnight; filter with suction, wash three times with absolute ethanol and acetone, wash once with ether, dry in vacuum, weigh and analyze the content of Polyporus umbellatus polysaccharide.

[0010] As a preferred solution, the process steps of the present invention are:

[0011] During the water extraction process, the mesh number of Polyporus umbellatus is 30 meshes, the ratio of material to water is 1:8, and the extraction time is 8h;

[0012] During the alcohol precipitation process, the volu...

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Abstract

The invention relates to a process for extracting polysaccharide, in particular to a process for extracting polysaccharide from umbellate pore fungus. The process for extracting polysaccharide from the umbellate pore fungus has the advantages of short time, high extraction rate and adaption of Soxhlet extraction and comprises the following processing steps of: (1) crushing crude drugs of umbellate pore fungus, sieving 10 to 30 meshes by using a test sieve, adding distilled water with the ratio of materials to water being between 1:7 and 1:9 into umbellate pore fungus powder, and soaking the umbellate pore fungus powder for 24 hours; (2) extracting the obtained solution in the step (1) for 7 to 9 hours by using a Soxhlet extractor; (3) obtaining filtrate through suction filtration, wherein the ratio of the crude drugs to the water solution in the filtrate is between 1:1 and 1:3; (4) adding 65 to 95 percent of ethanol into the filtrate, wherein the ratio of materials to water is between 1:4 and 1:6; (5) standing for the obtained solution in the step (4) overnight; (6) performing suction filtration, cleaning the obtained solution for three times respectively by using absolute ethyl alcohol and acetone, and cleaning the obtained solution for one time by using ether; and (7) weighing the obtained product after vacuum drying and analyzing the content of the obtained polyporus umbellatus polysaccharide.

Description

Technical field: [0001] The invention relates to an extraction process of total polysaccharides, more specifically, to an extraction process of total polysaccharides in Polyporus umbellatus. Background technique: [0002] Polyporus umbellatus is the dry sclerotia of the polyporus plant fungus Polyporus umbellatus. It has the effect of distilling water and dampening. Polyporus polysaccharide is a water-soluble polysaccharide extracted from Poria cocos. It is an immune enhancer and has obvious anti-tumor effects. It is used for the adjuvant treatment of radiotherapy and chemotherapy for lung cancer, leukemia, liver cancer, etc. It can improve the patient's disease resistance and improve clinical symptoms. [0003] The extraction method of Polyporus umbellatus polysaccharide is mainly the water decoction extraction method, which not only takes a long time, but also has a low extraction rate. Summary of the invention: [0004] In view of the above-mentioned problems, the present invent...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61P37/04
Inventor 孔令敏
Owner 孔令敏
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