Mass spectrum-compatible protein argentation

Abstract

The invention relates to mass spectrum-compatible protein argentation on electrophoretic gel, which comprises the following steps of: 1) putting gel formed by the electrophoresis of a protein sample in fixing solution for fixing, staining by using staining solution; 2) decoloring by using decoloring solution, washing by using deionized water, and performing sliver staining by using silver nitrate solution; and 3) washing by using the deionized water, developing by using a developing agent, and stopping by using ethylene diamine tetraacetic acid (EDTA) solution. Compared with the conventional protein argentation, the protein argentation provided by the invention has high sensitivity, short required time and high mass spectrum compatibility, and is suitable for the study of high-flux proteomics. The invention relates to the field of protein staining, in particular to the mass spectrum-compatible protein argentation on the electrophoretic gel.

Description

technical field [0001] The invention relates to the field of protein staining, in particular to a protein silver staining method compatible with mass spectrometry on electrophoresis gel. Background technique [0002] In proteomics research, gel protein staining technology is a key technology connecting two-dimensional electrophoresis and downstream mass spectrometry analysis. The current state-of-the-art mass spectrometers are capable of analyzing trace proteins at the pg level. Conventional Coomassie brilliant blue staining and silver staining seriously restrict the development of proteomics due to factors such as low sensitivity. In the past 30 to 40 years, many new staining techniques have been developed, but there are still one or several disadvantages as follows: low sensitivity, poor reproducibility, high toxicity, poor compatibility with mass spectrometry, and high price. Finding new staining methods to adapt to the current advanced mass spectrometry technology has ...

AI Technical Summary

Problems solved by technology

Conventional Coomassie brilliant blue staining and silver staining seriously restrict the development of proteomics due to factors such as low sensitivity

In the past 30 to 40 years, many new staining techniques have been developed, but there are still one or several disadvantages as follows: low sensitivity, poor reproducibility, high toxicity, poor compatibility with mass spectrometry, high price, etc.

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