Pseudomonas stutzeri and application thereof to water body dephosphorization

A technology of Pseudomonas stutzeri and water body, which is applied in the field of Pseudomonas stutzeri and its phosphorus removal in water body, can solve the problems that have not received enough attention inside and outside, and there are few phosphorus-accumulating bacteria species, so as to achieve prevention and control Eutrophication of water body, strong environmental adaptability, and the effect of saving production costs

Inactive Publication Date: 2011-05-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still very few isolated phosphorus-accumulating bacteria, and the method of using efficient bacteria to remove phosphorus is still in the exploratory stage, which is still far from meeting the needs of practical applications.
Moreover, the removal of low-concentration phosphorus (≤1.0mg / L) has not received enough attention at home and abroad, so qualitative and quantitative methods are used to screen high-efficiency phosphorus-accumulating strains for low-concentration phosphorus removal, and to explore its further application in phosphorus. The application in removal has important theoretical and practical significance

Method used

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  • Pseudomonas stutzeri and application thereof to water body dephosphorization
  • Pseudomonas stutzeri and application thereof to water body dephosphorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Discovery of Pseudomonas stutzeri YG-26

[0026] 1. Isolation of strains

[0027] In September 2008, the water samples (surface sediments) of Taihu Lake in Wuxi City, Jiangsu Province were collected. On the way, the samples were placed in an ice box below 4°C. After taking them back to the laboratory, 5g samples were weighed and placed in a triangle containing 45mL of sterile water. In the bottle, shake at 28°C and 180rpm on a shaker for 1h. After the sample is evenly dispersed in sterile water, draw 0.5mL of the suspension into a test tube with 4.5mL of sterile water, mix well, and make a concentration of 10%.-1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 100 μL of the bacterial suspension of each concentration was drawn and spread on YG solid medium plate, three replicates for each sample, and cultured in a constant temperature incubator at 28°C for 3 days. The colonies with different morphology were streaked and purified and labeled as YG-1, YG-2 and so o...

Embodiment 2

[0039] Example 2. Phosphorus removal ability of immobilized Pseudomonas stutzeri YG-26 on natural wastewater

[0040] 1. Preparation of immobilized Pseudomonas stutzeri YG-26

[0041] Sodium alginate: purchased from Sinopharm Chemical Reagent Company, catalog number 30164428, molecular weight 222. Polyvinyl alcohol (PVA): purchased from Sinopharm Chemical Reagent Company, catalog number 30153083, the average degree of polymerization (DP) is 2400-2500. Solution A (pH 6.5): composed of water and solute; solute and its concentration are: sodium alginate 1g / 100mL, polyvinyl alcohol 2g / 100mL. Solution B (pH 6.5): composed of water and solute; solute and its concentration are: CaCl24g / 100mL, boric acid 5g / 100mL (volume percentage).

[0042] 1. Pseudomonas stutzeri YG-26 was inserted into YG liquid medium, and shaken at 180 rpm at 28°C and cultured to log phase (OD). 600 = 0.2).

[0043] 2. Take 20 mL of bacterial liquid, centrifuge (6000 rpm, 10 min) to collect the bacterial cel...

Embodiment 3

[0055] Example 3. Phosphorus removal ability of immobilized Pseudomonas stutzeri YG-26 on natural lake water

[0056] The natural lake water is taken from Taihu Lake, and the total phosphorus concentration is 0.2mg / L.

[0057] 1. Preparation of immobilized Pseudomonas stutzeri YG-26

[0058] Sodium alginate: purchased from Sinopharm Chemical Reagent Company, catalog number 30164428, molecular weight 222. Polyvinyl alcohol (PVA): purchased from Sinopharm Chemical Reagent Company, catalog number 30153083, the average degree of polymerization (DP) is 2400-2500. Solution A (pH 6.5): composed of water and solute; solute and its concentration are: sodium alginate 1g / 100mL, polyvinyl alcohol 2g / 100mL. Solution B (pH 6.5): composed of water and solute; solute and its concentration are: CaCl24g / 100mL, boric acid 5g / 100mL (volume percentage).

[0059] 1. Pseudomonas stutzeri YG-26 was inserted into YG liquid medium, and shaken at 180 rpm at 28°C and cultured to log phase (OD). 600 =...

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Abstract

The invention discloses a Pseudomonas stutzeri and application thereof to water body dephosphorization. The Pseudomonas stutzeri YG-26 provided by the invention has a preservation number of CGMCC No.4093. An immobilized bacterium can be obtained by using the YG-26 embedded by sodium alginate and polyvinyl alcohol. The strain YG-26 and the immobilized bacterium thereof can be used for maximumly removing phosphorus in sewage so as to effectively prevent and control the occurrence of water body nutrition enrichment. By using the strain or the immobilized bacterium provided by the invention to perform water body treatment, the removal ratio is high, the method is simple in operation, and the cost is low along with economical efficiency and environmental protection, so the strain or the immobilized bacterium can be widely applied to further treatment of the sewage, and have significant economic, environmental and social benefits.

Description

technical field [0001] The invention relates to a strain of Pseudomonas stutzeri and its application in removing phosphorus from water bodies. Background technique [0002] Eutrophication of water bodies has become one of the problems that seriously affect water quality in the world. In my country, eutrophication lakes have an area of ​​5,000 square kilometers, and lakes with eutrophication conditions have an area of ​​14,000 square kilometers. Therefore, the governance of eutrophication has become one of the key technical difficulties for domestic and foreign researchers to overcome. [0003] A large number of studies have shown that phosphorus is the limiting factor in causing water eutrophication. In August 2008, scientists from Canada and the United States jointly published an article in the Proceedings of the National Academy of Sciences (PNAS) based on 37 years of limnology experimental research, proposing that nitrogen control is not required for the governance of e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 袁红莉李海峰杨金水李宝珍
Owner CHINA AGRI UNIV
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