Protein BnLEC1 related to seed fatty acid synthesis as well as encoding gene and applications thereof

A coding gene and gene technology, applied to the protein BnLEC1 related to the synthesis of seed fatty acids and its coding gene and application field, can solve the problem of changing fatty acid content, etc.

Active Publication Date: 2011-05-25
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above research results show that fatty acid metabolism in plants is a complex and highly coordinated process, and the genetic

Method used

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  • Protein BnLEC1 related to seed fatty acid synthesis as well as encoding gene and applications thereof
  • Protein BnLEC1 related to seed fatty acid synthesis as well as encoding gene and applications thereof
  • Protein BnLEC1 related to seed fatty acid synthesis as well as encoding gene and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, the acquisition of transgenic rapeseed with high seed oil content

[0050] 1. Acquisition and detection of promoter

[0051] 1. Acquisition of the promoter

[0052] According to the nucleotide sequence of napin A promoter (GenBank number: J02798.1), according to figure 2 Design primers for napin A promoter missing fragment (F is the front primer, R is the reverse primer), which is used to amplify the promoter specifically expressed in the seed embryo of Brassica napus L. The sequence is as follows: (the underline is the restriction site, and the first three bases are the protection bases))

[0053] D0 F: 5′-CGA AAGCTT TCTTCATCGGTGATTGATTC-3′;

[0054] D7 F: 5′-CGA AAGCTT CCATGCCAGAACATTAGCTACACG-3′;

[0055] D6 F: 5′-CGA AAGCTT AAGAATCGTTCATAAGATGCCATGC-3′;

[0056] D4 F: 5′-CGA AAGCTT TTTTAATTTTATGAAGTTAAGTTT-3′;

[0057] napinA R: 5′-TTA GTC GAC TCGTGTATGTTTTTAATCTTG-3'.

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Abstract

The invention discloses a protein related to seed fatty acid synthesis as well as an encoding gene and the applications thereof. The protein is sourced from a protein in (1) or (2): (1) a protein formed by amino acid sequences shown in a sequence (1) in a sequence table; and (2) a protein which is formed by substituting and/or deleting and/or adding one or more amino acid residues in an amino acid residue sequence in a sequence (1) in the sequence table, related to seed fatty acid synthesis and derived from the protein in (1). The encoding gene of the protein is transferred into plants to improve oil content in rape seeds. The invention has important theoretical and practical meanings to improving the fatty acid content and the related characters of plants (particularly oil plants) and has wide application and market prospects in the agriculture field.

Description

technical field [0001] The invention relates to a protein related to the synthesis of seed fatty acid, its coding gene and application. Background technique [0002] Fatty acids in plants are also known as vegetable oils, which are widely found in the seeds of plants. Vegetable oil is widely used, and the use of biotechnology to improve the composition and content of fatty acids in plants has important economic value. Among them, the operating gene selected by genetic engineering technology must be the key gene in the process of fatty acid biosynthesis in plants, but people still don't know much about the molecular regulation mechanism of plant fatty acid biosynthesis. Quantitative work is currently progressing slowly. Taking the dicot model plant Arabidopsis thaliana as an example, the embryogenesis of plants is mainly divided into two stages: early morphogenesis and late maturation. The entire embryonic development process is accompanied by the accumulation of storage s...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/11C12N15/63C12N15/82C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00A01H5/10
Inventor 左建儒谭河林杨晓辉牟金叶付福友
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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