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Preparation method of organic iron enzyme preparation

An iron enzyme and preparation technology, which is applied in the field of preparation of organic iron enzyme preparations, can solve the problems of low conversion rate, affect the use effect, etc., and achieve the effects of low production cost, enhanced disease resistance, and enhanced and anti-stress effects.

Inactive Publication Date: 2011-06-08
滨州市正元畜牧发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this preparation method usually only uses one kind of bacterium to carry out one conversion, and the conversion rate of inorganic iron into organic iron is low, only 30%-60%, which affects the use effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] A preparation method of an organic iron enzyme preparation, said method is, according to 81 parts by weight of bran, 13 parts by weight of soybean meal, 0.9 part by weight of rice husk powder, 0.6 part by weight of ammonium sulfate, 0.4 part by weight of potassium dihydrogen phosphate, malt 4 parts by weight, a proportion of 110 parts by weight of ferrous sulfate solution with a concentration of 6.5% by mass. Mix the above materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. , so that the water content is 65% by weight, and then, under the condition of 0.18MPa and 125°C, carry out high-temperature and high-pressure sterilization for 43 minutes. After the sterilization, when the above-mentioned materials are cooled to 31°C, Inoculate the aspergillus strain of 2.3% of its weight of Candida utilis (Candida utilis), stir it and cultivate it at a temperature of 29°C for 31 hou...

Embodiment 2

[0011] A preparation method of an organic iron enzyme preparation, said method is, according to 85 parts by weight of bran, 11 parts by weight of soybean meal, 1.1 parts by weight of rice husk powder, 0.4 parts by weight of ammonium sulfate, 0.6 parts by weight of potassium dihydrogen phosphate, malt 2 parts by weight, the ratio of 90 parts by weight of ferrous sulfate solution with a concentration of 6.9% by mass. Mix the above materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. , so that the water content is 55% by weight, then, under the conditions of 0.22MPa and 115°C, carry out high-temperature and high-pressure sterilization for 46 minutes. After the sterilization, when the above-mentioned materials are cooled to 29°C, Inoculate the aspergillus strain of 2.7% of its weight of Candida utilis (Candida utilis), stir and cultivate at a temperature of 27°C for 33 hours, then t...

Embodiment 3

[0013] A preparation method of an organic iron enzyme preparation, said method is, according to 83 parts by weight of bran, 12 parts by weight of soybean meal, 1.0 part by weight of rice husk powder, 0.5 part by weight of ammonium sulfate, 0.5 part by weight of potassium dihydrogen phosphate, malt 3 parts by weight, a proportion of 100 parts by weight of ferrous sulfate solution with a concentration of 6.7% by mass. Mix the above materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. , so that the water content is 60% by weight, then, under the condition of 0.20MPa and 120°C, carry out high-temperature and high-pressure sterilization for 45 minutes, after the sterilization, when the above-mentioned materials are cooled to 30°C, Inoculate the aspergillus strain of 2.5% of its weight of Candida utilis (Candida utilis), stir it and cultivate it at a temperature of 28°C for 32 hours, ...

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Abstract

The invention provides a preparation method of an organic iron enzyme preparation, which comprises the steps of: uniformly mixing raw materials, such as wheat bran, bean pulp, rice husk powder, a ferrous sulfate solution and the like to prepare a mixture, carrying out high-temperature high-pressure sterilization, inoculating a koji tray strain of candida utilis after sterilizing, uniformly stirring and then culturing, then taking materials obtained by the culturing out, inoculating a koji tray strain of aspergillus usamii, uniformly stirring and then subculturing, then taking materials obtained by the subculturing out, inoculating a koji tray strain of bacillus subtilis, uniformly stirring and then culturing for the third time, and drying and crushing materials obtained by the third-time culturing to prepare the organic iron enzyme preparation. The method has the advantages of simple preparation process and low production cost; three times of transformation are continually carried out by adopting the three stains, and inorganic iron is transformed into organic iron with high transformation rate reaching above 98 percent; and the prepared enzyme preparation integrates organic trace elements, an enzyme preparation and probiotics into a whole, has multiple biological functions, and can enhance the disease-resistant capacity and the stress-resisting function on a fed animal.

Description

technical field [0001] The invention provides a preparation method of an organic iron enzyme preparation. Background technique [0002] As a new feed nutritional additive, organic iron has good biological functions. There are currently two types of organic iron: complexed iron and chelated iron. Complex iron is composed of a central ion (or atom) such as F e 2+ A complex ion or molecule formed by covalently bonding with a ligand. Chelated iron is a special kind of complexed iron, which is a complexed iron with a ring structure formed by a coordination reaction between one or more groups and a metal ion. The production process of these organic irons is complicated and the production cost is relatively high. Now there is a preparation method of an organic iron enzyme preparation, which uses probiotics such as fungi and bacteria as carriers to convert inorganic iron into organic iron through biotransformation, thereby increasing the potency. However, this preparation meth...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K20/189
Inventor 吴希恩
Owner 滨州市正元畜牧发展有限公司
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