Macro fungi insecticide for preventing and controlling aromia bungii
A fungal pesticide and a large-scale technology, which are applied in the field of microbial source large-scale fungal pesticides and their preparation, can solve the problems of short drug effect time, strong pertinence and low lethality, and achieve high temperature resistance in drug effect time and high drug resistance. Long effective time and good control effect
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Embodiment 1
[0045] A macrofungal insecticide for preventing and controlling the pink-necked longhorn beetle. Its components and contents are: 40% crude pedopeptide toxinoids of P. Purpose fungus chaff powder.
[0046] A macrofungal insecticide for preventing and treating the pink-necked longhorn beetle, the preparation method of which is:
[0047] 1. Screening of macrofungi
[0048] One test tube each of the parent species of Amanita fruticosa and Amanita striata containing toxic peptide toxins was screened from large fungi.
[0049] 2. Shaker liquid submerged fermentation
[0050] (1), liquid medium: 200 grams of potatoes, 20 grams of glucose, NaNO 3 3 grams, KH 2 PO 4 1g, KCl0.5g, MgSO 4 ·7H 2 O 0.05 g, FeSO 4 0.01g, 1000ml distilled water, PH7.0.
[0051] (2) Take two triangular flasks with a capacity of 1000 ml, label them as bottle A and bottle B, and then add 500 ml of liquid medium to bottle A and bottle B respectively. Sterilize at 121°C and 0.1 MPa for 15 minutes.
[0...
Embodiment 2
[0063] A macrofungal insecticide for preventing and controlling the pink-necked longhorn beetle. Its components and contents are: 40% crude pedopeptide toxinoids of P. Purpose fungus chaff powder.
[0064] A macrofungal insecticide for preventing and treating the pink-necked longhorn beetle, the preparation method of which is:
[0065] 1. Screening of macrofungi
[0066] One test tube each of the parent species of Amanita fruticosa and Amanita striata containing toxic peptide toxins was screened from large fungi.
[0067] 2. Shaker liquid submerged fermentation
[0068] (1), liquid medium: 200 grams of potatoes, 20 grams of glucose, NaNO 3 3 grams, KH 2 PO 4 1g, KCl0.5g, MgSO 4 ·7H 2 O0.05 g, FeSO 4 0.01g, 1000ml distilled water, PH7.0.
[0069] (2) Take two triangular flasks with a capacity of 500 ml, label them, and mark them as bottle A and bottle B, and then add 200 ml of liquid culture medium to bottle A and bottle B respectively. Sterilize at 126°C, 0.1 MPa for...
Embodiment 3
[0081] A macrofungal insecticide for preventing and controlling the pink-necked longhorn beetle. Its components and contents are: 40% crude pedopeptide toxinoids of P. Purpose fungus chaff powder.
[0082] A macrofungal insecticide for preventing and treating the pink-necked longhorn beetle, the preparation method of which is:
[0083] 1. Screening of macrofungi
[0084] One test tube each of the parent species of Amanita fruticosa and Amanita striata containing toxic peptide toxins was screened from large fungi.
[0085] 2. Shaker liquid submerged fermentation
[0086] (1), liquid medium: 200 grams of potatoes, 20 grams of glucose, NaNO 3 3 grams, KH 2 PO 4 1g, KCl0.5g, MgSO 4 ·7H 2 O 0.05 g, FeSO 4 0.01g, 1000ml distilled water, PH7.0.
[0087] (2) Take two triangular flasks with a capacity of 1000 ml, label them as bottle A and bottle B, and then add 600 ml of liquid medium to bottle A and bottle B respectively. Sterilize at 123°C, 0.1 MPa for 18 minutes.
[0088...
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