Colloidal gold test strip for double-amplification system and preparation method thereof

Abstract

The invention belongs to the field of medical clinical immediate tests, and relates to a colloidal gold test strip for a double-amplification system and a preparation method thereof. The colloidal gold test strip for the double-amplification system comprises a test strip bottom lining, and a sample pad, a polyester film coated with a gold-labeled antibody, a coating film and absorbent paper whichare lapped with and adhered to the test strip bottom lining in turn, wherein the middle part of the coating film is parallelly provided with a detection line coated with streptavidin and an F(ab)2 fragment of a monoclonal antibody of an antigen to be detected, and a control line coated with a rabbit anti-rat immunoglobulin G (IgG) antibody; the F(ab)2 fragment of the monoclonal antibody of the antigen to be detected and biotin are coated in the sample pad; and the gold-labeled antibody is colloidal gold labeled protein A and the monoclonal antibody of the antigen to be detected. The test strip has the advantages of high specificity, sensitivity and detection speed, has low cost, is easy to operate, can be widely applied to the detection of the antigen to be detected, and lays a foundationfor the timely discovery and prediction and effective detection of diseases, and an instrument and equipment are not required to be used.

Description

technical field

[0001] The invention belongs to the field of instant medical clinical testing, and relates to a colloidal gold test strip with a dual amplification system and a preparation method thereof, in particular to a colloidal gold test strip for detecting cardiac troponin I and a preparation method thereof. Background technique

[0002] At present, 17 million people die from cardiovascular diseases every year in the world, and more than half of them die from acute myocardial infarction. , and there is a trend of "younger" patients, cardiovascular and cerebrovascular diseases have become the main cause of death in my country, especially the incidence of ischemic myocardial injury caused by myocardial infarction is increasing year by year. Therefore, early diagnosis and active treatment of myocardial injury are very important. Experts pointed out that seizing the "golden time" 6 hours after the onset of chest pain is the key to treating patients' lives. Because of its o...

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