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Colloidal gold test strip for double-amplification system and preparation method thereof

A technology of colloidal gold test paper and amplification system, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problem of not further improving the sensitivity of colloidal gold, and achieve the effect of enhancing binding force, improving specificity, and improving sensitivity

Active Publication Date: 2011-06-22
GETEIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the sensitivity problem of colloidal gold, there is no method to further improve the sensitivity of colloidal gold

Method used

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  • Colloidal gold test strip for double-amplification system and preparation method thereof
  • Colloidal gold test strip for double-amplification system and preparation method thereof
  • Colloidal gold test strip for double-amplification system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Such as figure 1 As shown, a colloidal gold test strip of a double amplification system comprises a test strip bottom liner 1, and a sample pad 2 which is overlapped and pasted successively on the test strip bottom liner 1, and a polymer coated with a gold-labeled antibody. Ester film 3, coating film 4 and absorbent paper 5, coating film 4 is coated with detection line 6 and control line 7, and the middle part of coating film 4 is provided with a monoclonal antibody coated with streptavidin-cardiac troponin I in parallel F(ab) of 4C2 2 Fragment test line 6 and a control line 7 coated with rabbit anti-mouse IgG antibody, sample pad 2 coated with F(ab) of mouse cardiac troponin I monoclonal antibody 4C2 2 Fragment-biotin, the gold-labeled antibody is ProteinA labeled with gold particles, through the non-specific binding of ProteinA to the Fc segment of the cardiac troponin I monoclonal antibody M155, the amplified indirect binding of the cardiac troponin I monoclonal ant...

Embodiment 2

[0045] Embodiment 2 adopts the concrete preparation method of the colloidal gold test paper strip of conventional method as follows:

[0046] Cardiac troponin I monoclonal antibody 4C2 and M155 (paired antibody), both purchased from Fitzgerald.

[0047] Preparation of coating film:

[0048] 1) Preparation of coating buffer: filter 0.025M PBS with pH 7.4 through a 0.22u membrane, store at 4°C for use, and have a validity period of 7 days.

[0049] 2) Preparation of blocking solution: PBS containing 1% BSA, 1% sucrose, 0.025M, pH 7.5 was filtered through a 0.22U membrane and placed at 4°C for later use. The validity period was 3 days.

[0050] 3) F(ab) of monoclonal antibody 4C2 2 Fragment preparation: Digest the monoclonal antibody with trypsin, pass through the SPA-SepharoseCL-4B gel chromatography column to remove the Fc fragment, and collect F(ab) 2 fragment.

[0051] 4) F(ab) 2 Preparation of the detection line 6 in section 6: the antibody was prepared at a concentrati...

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Abstract

The invention belongs to the field of medical clinical immediate tests, and relates to a colloidal gold test strip for a double-amplification system and a preparation method thereof. The colloidal gold test strip for the double-amplification system comprises a test strip bottom lining, and a sample pad, a polyester film coated with a gold-labeled antibody, a coating film and absorbent paper whichare lapped with and adhered to the test strip bottom lining in turn, wherein the middle part of the coating film is parallelly provided with a detection line coated with streptavidin and an F(ab)2 fragment of a monoclonal antibody of an antigen to be detected, and a control line coated with a rabbit anti-rat immunoglobulin G (IgG) antibody; the F(ab)2 fragment of the monoclonal antibody of the antigen to be detected and biotin are coated in the sample pad; and the gold-labeled antibody is colloidal gold labeled protein A and the monoclonal antibody of the antigen to be detected. The test strip has the advantages of high specificity, sensitivity and detection speed, has low cost, is easy to operate, can be widely applied to the detection of the antigen to be detected, and lays a foundationfor the timely discovery and prediction and effective detection of diseases, and an instrument and equipment are not required to be used.

Description

technical field [0001] The invention belongs to the field of instant medical clinical testing, and relates to a colloidal gold test strip with a dual amplification system and a preparation method thereof, in particular to a colloidal gold test strip for detecting cardiac troponin I and a preparation method thereof. Background technique [0002] At present, 17 million people die from cardiovascular diseases every year in the world, and more than half of them die from acute myocardial infarction. , and there is a trend of "younger" patients, cardiovascular and cerebrovascular diseases have become the main cause of death in my country, especially the incidence of ischemic myocardial injury caused by myocardial infarction is increasing year by year. Therefore, early diagnosis and active treatment of myocardial injury are very important. Experts pointed out that seizing the "golden time" 6 hours after the onset of chest pain is the key to treating patients' lives. Because of its o...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/532
Inventor 苏恩本王勇王路海陈伟
Owner GETEIN BIOTECH
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