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Triterpenoid-based compound used as a virus inhibitor

A compound and composition technology, applied in antiviral agents, plant raw materials, active ingredients of aldehydes, etc.

Inactive Publication Date: 2011-06-29
RNL BIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Japanese alder extracts have only limited use as they have the disadvantage of showing antiviral activity only when administered at high concentrations

Method used

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  • Triterpenoid-based compound used as a virus inhibitor
  • Triterpenoid-based compound used as a virus inhibitor
  • Triterpenoid-based compound used as a virus inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Embodiment 1: the preparation of Japanese alder extract

[0100] 3.5 kg of Japanese alder (RNL BIO Co., Ltd.) bark was added to 9 L of 95% ethanol, sonicated three times at 55 °C, and then concentrated to obtain 900 g of ethanol fraction (12B-AJ-5A). Such as figure 1 As shown, the obtained 12B-AJ-5A component is CH 2 Cl 2 and ethanol to obtain dichloromethane (CH 2 Cl 2 ) component (12B-AJ-5B, 139 g), ethanol component (12B-AJ-5C, 400 g) and water component (12B-AJ-5D).

[0101] Furthermore, 12B-AJ-5D was treated with 20%, 50%, 70% and 100% methanol to obtain 12B-AJ-5E, 12B-AJ-5F, 12B-AJ-5G and 12B-AJ-5H .

Embodiment 2

[0102] Example 2: Measurement of antiviral activity of Japanese alder extract

[0103] To measure the antiviral activity of the Japanese alder extract and compounds derived from the Japanese alder extract, KBNP-0028 (KCTC 10866BP), which has excellent proliferation ability, was used as an avian influenza virus. Herein, KBNP-0028 (KCTC 10866BP) was obtained by subculturing A / chicken / Korea / SNU0028 / 2000 (H9N2) isolated in Korea in 2000 and cloning the cultured virus.

[0104] For incubation of hatchery eggshell fragments, the shells of 10–11 day old SPF hatchery eggs (Sunrise Co., NY) were washed with 70% ethanol, and embryos and body fluids were removed. The resulting eggshells were cut to approximately 8 mm x 8 mm size so that the chorioallantoic membrane attached to the inner surface of the eggshell was not detached. Add the cut eggshell fragments to each well of a 24-well culture plate. The medium used in this experiment was prepared by mixing 199 medium (GIBCO-BRL, NY, U...

Embodiment 3

[0110] Example 3: Measurement of Cytotoxicity of Japanese Alder Extracts

[0111] In order to detect whether the Japanese alder extract has cytotoxicity, each organic solvent component prepared in Example 1 (12B-AJ-5A, 12B-AJ-5B, 12B-AJ-5C, 12B-AJ-5D, 12B-AJ-5E, 12B-AJ-5F, 12B-AJ-5G and 12B-AJ-5H, each having a concentration of 12.5, 25, 50 and 100 μg / mL) were added to the MTT solution (0.5% MTT in water) . Then, each solution was added to each well of a 96-well plate in which chicken embryo fibroblasts (CEF) had been cultured. Then, each well of the plate was incubated at 37° C. for 1-3 hours, and then 120 μL of DMSO was added thereto and stirred for 30 minutes. Then, the absorbance of each well was measured at a wavelength of 562 nm using a microplate reader. As a result, 12B-AJ-5A and 12B-AJ-5B fractions showed relatively high cytotoxicity, 12B-AJ-5C fraction showed moderate cytotoxicity, 12B-AJ-5D, 12B-AJ-5E, 12B-AJ- 5F, 12B-AJ-5G and 12B-AJ-5H showed relatively low ...

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Abstract

The present invention relates to an application for a triterpenoid-based compound of Chemical formula (1), for suppressing viral activity. The triterpenoid-based compound according to the present invention is outstandingly effective in suppressing viral activity and can therefore be beneficially used as a therapeutic agent for medical conditions in which a virus is involved.

Description

technical field [0001] The present invention relates to the use of triterpenoids of general formula (1) to inhibit virus activity. Background technique [0002] Viruses cause various diseases, and in particular, a typical causative virus causing problems in the field of animal husbandry is avian influenza virus. Avian influenza viruses belong to the family of orthomyxoviruses and cause severe damage to poultry such as hens and turkeys. Avian influenza viruses are divided into highly pathogenic avian viruses, low pathogenic avian viruses and non-pathogenic avian viruses according to their pathogenicity. The highly pathogenic avian virus is classified as a "category A virus" by the International Office of Epizootics (OIE), and is referred to as a category I infectious livestock disease in Korea. [0003] Influenza viruses are divided into three types, A, B, and C, according to the antigenic properties of their matrix proteins and nucleocapsid proteins. In addition, accordin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56
CPCA61K31/11A61P31/12A61P31/16A61K31/565A61K36/185A61K31/56
Inventor 罗廷灿金荣镐权赫俊恽胡彤
Owner RNL BIO
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