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Bird nest fern spore germination breeding method

A technology for spore germination and bird's nest fern, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low spore germination rate, difficulty in differentiation and emergence, slow recovery, etc., and achieves the effect of shortening the cultivation time.

Active Publication Date: 2012-11-28
DASHUN INT FLOWER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bird's nest fern is usually propagated by division and spore sowing. Division reproduction is simple and easy, but it is easy to damage the plant shape, recovery is slow and is limited by seasons; the spore germination rate is very low during spore sowing, it is difficult to differentiate and emerge, and it is not easy to obtain A large number of seedlings
At present, there are also tissue culture techniques for propagation, but the cost is high and the culture procedures are cumbersome.

Method used

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  • Bird nest fern spore germination breeding method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0010] Example 1 (test group)

[0011] Wrap the collected bird's nest fern spores with gauze, perform aseptic treatment, add sterile water to make a sterile spore suspension with a density of 1500-2000 / ml; mix the above sterile spore suspension with the initial culture The medium was mixed at a ratio of 1:4, and the primary medium was added with 0.2mg GA per liter 3 , sucrose 20g, agar 6g, MS medium with a pH of 5.8-6.0; after mixing evenly, pour it on a plate, the thickness of the plate is 3mm, after solidification, cut into thin slices containing 600-800 spores each; put the above thin slices into Cultivate in a petri dish of MS liquid medium, the culture conditions are temperature 23~25°C, light intensity 1500~2000Lx, light 16 hours a day, until the sporophyte seedlings are formed; after the sporophyte seedlings are formed, transfer the sporophyte seedlings to each Add 30g sucrose to 1 liter, continue to cultivate in MS liquid medium with pH of 5.8-6.0, and change the medi...

example 2

[0012] Example 2 (test group)

[0013] Wrap the collected bird's nest fern spores with gauze, perform aseptic treatment, add sterile water, and make a sterile spore suspension with a density of 1500-2000 / ml; mix the above sterile spore suspension with the initial The medium was mixed at a ratio of 1:4, and the initial medium was added with 0.5mg GA per liter 3 , 60g of sucrose, 6g of agar, MS medium with a pH of 5.8-6.0; after mixing evenly, pour it on a plate, the thickness of the plate is 5mm, after solidification, cut into thin slices containing 600-800 spores each; put the above thin slices into Cultivate in a petri dish of MS liquid medium, the culture conditions are temperature 23~25°C, light intensity 1500~2000Lx, light 16 hours a day, until the sporophyte seedlings are formed; after the sporophyte seedlings are formed, transfer the sporophyte seedlings to each Add 30g sucrose to 1 liter, continue to cultivate in MS liquid medium with pH of 5.8-6.0, and change the medi...

example 3

[0014] Example 3 (test group)

[0015] Wrap the collected bird's nest fern spores with gauze, perform aseptic treatment, add sterile water, and make a sterile spore suspension with a density of 1500-2000 / ml; mix the above sterile spore suspension with the initial The medium was mixed at a ratio of 1:4, and the initial medium was added with 1.0mg GA per liter 3 , sucrose 30g, agar 6g, MS medium with a pH of 5.8-6.0; after mixing evenly, pour it on a plate, the thickness of the plate is 4mm, after solidification, cut into thin slices containing 600-800 spores each; put the above thin slices into Cultivate in a petri dish of MS liquid medium, the culture conditions are temperature 23~25°C, light intensity 1500~2000Lx, light 16 hours a day, until the sporophyte seedlings are formed; after the sporophyte seedlings are formed, transfer the sporophyte seedlings to each Add 30g sucrose to 1 liter, continue to cultivate in MS liquid medium with pH of 5.8-6.0, and change the medium eve...

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Abstract

The invention relates to a bird nest fern spore germination breeding method which comprises the following steps of: wrapping a collected bird nest fern spore by using gauze, carrying out asepsis treatment and adding sterile water for blending into sterile spore turbid liquid containing 1,500-2,000 sterile spores per milliliter; mixing the sterile spore turbid liquid with an initial culture mediumaccording to the proportion of 1:4, wherein the initial culture medium is an MS culture medium in which 0.2-1.0mg of GA3, 20-60g of cane sugar and 6g of agar are added in each liter; after uniformly mixing, reserving a flat plate, wherein the thickness of the flat plate is 3-5mm; after solidifying, cutting into a sheet containing 600-800 spores in each part; charging the sheet into a culture dishin which an MS liquid culture medium is added for culturing, wherein the culture condition comprises the temperature of 23-25 DEG C and the illumination intensity of 1,500-2,000Lx; and illuminating for 16 hours each day till a spore seedling is formed.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation, in particular to the technical field of fern plant propagation. Background technique [0002] Bird's Nest Fern (Neottopteris nidus) is a large perennial evergreen epiphyte. Leaf clusters grow on the outer edge of the top of the rhizome, arranged radially around, and the center of the leaf cluster is empty like a bird's nest. The sporangia are born on the upper side of the lateral veins and extend to 1 / 2 of the leaves. Native to tropical and subtropical regions. It likes warmth, dampness, and is not cold-resistant. It should be loose, well-drained and water-retaining soil. Bird's nest fern is usually propagated by division and spore sowing. Division reproduction is simple and easy, but it is easy to damage the plant shape, recovery is slow and is limited by seasons; the spore germination rate is very low during spore sowing, it is difficult to differentiate and emerge, and it is not ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨铁顺
Owner DASHUN INT FLOWER