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Tissue culture method of myrica rubra

A technology of tissue culture and culture medium, applied in the field of plant tissue culture, can solve the problems of low tissue culture pollution rate, bayberry tissue culture pollution rate, impracticability and other problems, and achieve the effect of breaking through high pollution rate and breaking through technical barriers

Inactive Publication Date: 2011-07-13
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to propose a kind of explant sterilization effect is better, the tissue culture pollution rate is lower for conventional method sterilization easily causes the pollution rate of bayberry tissue culture to be as high as 80~90%, difficult problem that can't be implemented in production Method for tissue culture of bayberry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: (red bayberry tissue culture method 1)

[0030] (1) The preparation of the medium, including the basic medium and the components of the medium at each stage, and the weight of each component in each liter of medium:

[0031] 1) Basic medium: WPM is used as the basic medium for initial induction, shoot elongation and proliferation, in which sucrose is 30 g / L, agar powder is 7 g / L, pH is 5.6; rooting is 1 / 2 MS is the basic medium, the sucrose in the medium is 20 g / L, the agar powder is 6 g / L, and the pH is 5.6;

[0032] The WPM medium formula is: WPM medium formula: macroelement: ammonium nitrate (NH 4 NO 3 ) 400 mg / L, potassium sulfate (K 2 SO 4 ) 900 mg / L, potassium dihydrogen phosphate (KH 2 PO 3 ) 170 mg / L, magnesium sulfate (MgSO 4 ?7H 2 O) 370 mg / L; calcium salt: calcium chloride (CaCl 2 ?2H 2 O) 96 mg / L; calcium nitrate tetrahydrate (Ca (NO 3 ) 2 ?4H 2 O) 556 mg / L; trace elements: boric acid (H 3 BO 3 ) 6.2 mg / L, manganese sulfate (MnS...

Embodiment 2

[0043] Embodiment 2: (red bayberry tissue culture method 2)

[0044] In this example, step (1) medium preparation: 1) Basic medium: Among them, the sucrose in WPM medium is 30 g / L, the agar powder is 6.5 g / L, and the pH is 5.7; 1 / 2 MS medium Medium sucrose is 20 g / L, agar powder is 6 g / L, pH is 5.6; 2) Initial induction medium Y1: WPM+6-BA 0.05 mg / L+NAA 0.01 mg / L+PVP 0.9 g / L; 3) Shoot elongation and proliferation medium Y2: WPM+6-BA 0.5 mg / L+NAA 0.45 mg / L+GA 3 0.35 mg / L+PVP 0.9 g / L; 4) Rooting medium Y3: 1 / 2 MS+IBA 0.5 mg / L, pH 5.6;

[0045] Step (2) Cultivation of aseptic seedlings of red bayberry: 1) Selection and sterilization of explants: Soak the explant stems in 70% alcohol and shake for 28 s; then in 0.1% HgCl 2 Soak in the solution and shake to sterilize for 7.5 min; 2) Initial induction culture: culture for 13 days at 24°C, light intensity 2500 Lx, light intensity 12 h / d; 3) Shoot elongation culture: grow at 24°C, light intensity 2500 Lx, light 12 h / d, cultured fo...

Embodiment 3

[0046] Embodiment 3: (waxberry tissue culture method 3)

[0047] In this example, the preparation of the medium in step (1): 1) Basic medium: Among them, the sucrose in the WPM medium is 30 g / L, the agar powder is 6 g / L, and the pH is 5.8; 1 / 2 MS medium Medium sucrose 20 g / L, agar powder 6 g / L, pH 5.6; 2) Initial induction medium Y1: WPM+PVP 1.0 g / L; 3) Shoot elongation and proliferation medium Y2: WPM+6 -BA 0.5 mg / L+NAA 0.5 mg / L+GA 3 0.3 mg / L+PVP 1.0 g / L; 4) Rooting medium Y3: 1 / 2 MS+IBA 0.5 mg / L, pH 5.6;

[0048] Step (2) Cultivation of aseptic seedlings of red bayberry: 1) Selection and sterilization of explants: Soak the explant stems in 70% alcohol and shake for 25 s; then in 0.1% HgCl 2 Soak in the solution and shake to sterilize for 8 min; 2) Initial induction culture: cultured for 15 days at 26°C, light intensity 2000 Lx, light intensity 12 h / d; 3) Shoot elongation culture: at 26°C, light intensity 2000 Lx, light intensity 12 h / d and cultured for 21 days; 4) Prolif...

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Abstract

The invention discloses a tissue culture method of myrica rubra, belonging to the technical field of tissue culture of plants. The method comprises the following steps of: (1) preparing a culture medium; (2) culturing the sterile seedlings of the myrica rubram; and the like. Aiming at the characteristics of the branches of the myrica rubra, the invention provides a prominent and efficient sterilizing method to reduce the pollution rate of the explant of the myrica rubra to 40-50% from 80-90% before improvement so as to successfully build the culture system of the sterile seedlings of the myrica rubra; the method effectively breaks through the technical obstruct that the explant of the myrica rubra tissue culture has high pollution rate, and lays the foundation of improvement of the current myrica rubra culture variety. The method disclosed by the invention can be widely used in the breed improvement research of the myrica rubra and fast reproducing the good variety.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for culturing waxberry tissue. Background technique [0002] Bayberry ( Myrica Rubra Sieb. & Zucc.) is an important fruit tree species in southern my country. The fruit is sweet and sour and has a unique flavor. In recent years, bayberry planting area has developed rapidly, and the country has exceeded 200,000 ha at present, and the benefits are remarkable, and it has become the main source of income for farmers in some mountainous areas. However, there is a serious situation of a single type of variety in the production of red bayberry, and the varieties that have been promoted for a long time all come from local varieties, or propagate from preferred single plants. [0003] Tissue culture and the mutagenesis technology system established on the basis of it are important platforms for efficient breeding of fruit trees and improvement of production specie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 谢小波戚行江求盈盈郑锡良项康华周鑫
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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