Method for quickly breeding Litsea coreana test tube plantlets by spores

A technology of test-tube seedlings and eagle tea, applied in gardening methods, botanical equipment and methods, gardening, etc., to achieve the effect of fast speed and simple operation

Inactive Publication Date: 2011-07-20
宁国市亿邦生物科技有限公司
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Problems solved by technology

[0004] The method of tissue culture for propagation refers to a culture technique that uses an aseptic method to make the isolated organs, tissues and

Method used

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Embodiment Construction

[0017] This embodiment includes the following steps:

[0018] Step 1. Take the branches of Eagle Tea between the beginning of autumn and the frost (the branches in spring have heavy endophytic bacteria, and the branches in summer are heavily infected with bacteria, so they are not suitable for explants). Put the branches in the refrigerator at 4°C for 1-3 days, and then take the branches The spores on the surface were washed with running water for 120 minutes, and then the explants were disinfected; the surface disinfection method was first treated with 75% alcohol for 30 seconds, then shaken with 0.1% mercury liter for 8 minutes, and rinsed with sterile water for 3 times.

[0019] Step 2: Peel off the outer skin of the spores under aseptic conditions to retain the base and endothelium of the spores, and inoculate the peeled spores in the starting medium for cultivation. The starting medium is MS as the basic medium, and then add 2mg / L6-BA, 0.2mg / L NAA, 30mg / L sucrose, 2.6g / L ...

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Abstract

The invention discloses a method for quickly breeding Litsea coreana test tube plantlets by spores, comprising the following steps: taking one spore of the Litsea coreana as an explant; starting to cultivate to induce cluster buds; and carrying out branch bud proliferation cultivation, branch cultivation and rooting cultivation to obtain the Litsea coreana test tube plantlets. The method has the advantage of high speed and can be industrially produced, is convenient to operate and is suitable for industrially culturing plantlets, and the reproduction coefficient is 3-5 times.

Description

technical field [0001] The invention relates to asexual reproduction of plants, in particular to a method for rapidly propagating test-tube seedlings of the eagle tea tree through spore culture. Background technique [0002] Eagle tea tree, scientific name: Leopard skin camphor (Litsea coreana), is an evergreen tree plant belonging to Lauraceae and Xingia genus, with alternate leaves, very thick leaves, and dark green color. It is found in Sichuan, Guizhou, Yunnan, Anhui and other places in my country. sporadic distribution. The tea made from the tender leaves of the leopard skin camphor tree is called Eagle tea, which is a special tea. Eagle tea and its beverage series are rich in various plant essences, non-polluting, and good in flavor, and are popular among consumers. favored by those. As a wild tree species, the eagle tea tree is difficult to breed naturally. At present, the reproductive rate of the artificially cultivated seedlings is low, which seriously restricts th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邝荔香刘静於琦舒阿庆
Owner 宁国市亿邦生物科技有限公司
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